HPLC Separation of Amino Acids on Newcrom AH Column

HPLC Method for Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine on Newcrom AH by SIELC Technologies

Amino acids are the building blocks of proteins. Based on their dietary requirement, they are classified into essential and non-essential amino acids. Essential amino acids cannot be synthesized by the human body in sufficient quantities and must be obtained from the diet. Non-essential amino acids, on the other hand, can be synthesized by the body and are not dependent on dietary intake.

It’s worth noting that while these amino acids are considered “non-essential” for adults under normal circumstances because the body can synthesize them, there are situations where some may become “conditionally essential.” This means that under certain conditions like illness, stress, or trauma, the body might not produce them in sufficient quantities, and dietary intake becomes necessary. Arginine, for instance, is considered conditionally essential, especially during periods of rapid growth, illness, or trauma.

You can find detailed UV spectra of Aspartic Acid and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Glutamine and information about its various lambda maxima by visiting the following link.

Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine can be retained and analyzed using the Newcrom AH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Formic Acid buffer. Detection is performed using CAD.

 

Condition

Column Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 50%
Buffer  Formic Acid – 0.1%
Flow Rate 1.0 ml/min
Detection CAD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Aspartic Acid, L-Glutamine, Glutamic Acid, Glycine

 

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Aspartic Acid
Glutamic Acid
Glycine
L-Glutamine

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.