L-Isoleucine

CAS Number73-32-5
Molecular FormulaC6H13NO2
Molecular Weight131.175
InChI KeyAGPKZVBTJJNPAG-WHFBIAKZSA-N
LogP-0.720
Synonyms
  • 2S,3S)-?-Amino-?-methyl-n-valeric acid
  • (2S,3S)-?-Amino-?-methylvaleric acid
  • (S)-Isoleucine
  • (S,S)-Isoleucine
  • [S-(R*,R*)]-2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylvaleric acid
  • 2S,3S-Isoleucine
  • erythro-L-Isoleucine
  • Isoleucine
  • ISOLEUCINE, L-
  • L-(+)-Isoleucine
  • L-Isoleucin
  • L-isoleucina
  • L-Norvaline, 3-methyl-, erythro-
  • NSC 46708
  • Pentanoic acid, 2-amino-3-methyl-, (2S,3S)-
  • Secretory protein (human clone HCEEE79 precursor)
  • Acetic acid, amino-sec-butyl-
  • Acetic acid, amino(1-methylpropyl)-, (R*,R*)-
  • (S-(R*,R*))-2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylpentanoic acid, (S-(R*,R*))-
  • EINECS 200-798-2
  • Norvaline, 3-methyl-
  • Pentanoic acid, 2-amino-3-methyl-
  • Pentanoic acid, 2-amino-3-methyl-, (S-(R*,R))-
  • Valeric acid, 2-amino-3-methyl-
  • Isoleucina
  • Isoleucinum
  • UNII-04Y7590D77 FDA Registry Number
  • (2S,3S)-2-Amino-3-methylpentanoate
  • (2S,3S)-2-amino-3-methyl-Pentanoate
  • (2S,3S)-2-amino-3-methyl-pentanoic acid
  • (2S,3S)-a-Amino-b-methyl-N-valerate
  • (2S,3S)-a-Amino-b-methyl-N-valeric acid
  • (2S,3S)-a-Amino-b-methylvalerate
  • (2S,3S)-a-Amino-b-methylvaleric acid
  • (2S,3S)-alph-Amino-beta-methylvalerate
  • (2S,3S)-alph-Amino-beta-methylvaleric acid
  • (2S,3S)-alpha-Amino-beta-merthyl-N-valerate
  • (2S,3S)-alpha-Amino-beta-merthyl-N-valeric acid
  • (2S,3S)-alpha-Amino-beta-merthylvalerate
  • (2S,3S)-alpha-Amino-beta-merthylvaleric acid
  • (2S,3S)-alpha-Amino-beta-methyl-N-valerate
  • (2S,3S)-alpha-Amino-beta-methyl-N-valeric acid
  • (2S,3S)-alpha-Amino-beta-methylvalerate
  • (2S,3S)-alpha-Amino-beta-methylvaleric acid
  • 2-Amino-3-methylpentanoate
  • 2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylvalerate
  • Iso-leucine

Applications:

HPLC Separation of Allo-Isoleucine, Isoleucine, Leucine on Primesep 100 Column

November 12, 2019

HPLC Method for D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine on Primesep 100 by SIELC Technologies

HPLC Separation of Allo-Isoleucine  Isoleucine, Leucine on Primesep 100 Column Chr_1197

High Performance Liquid Chromatography (HPLC) Method for Analysis of D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine 

Alloisoleucine is an amino acid with C6H13NO2 chemical formula. It is a key indicator when diagnosing Maple Syrup Urine Disease, when a body cannot metabolize amino acids in specific foods. Outside of that, it is also used in peptide synthesis and drug development.

Isoleucine is an amino acid with chemical formula C6H13NO2. It is a building block for proteins and is used in a variety of bodily functions. It helps form hemoglobin, regulating blood sugar, plays a key role in energy production, and increases the speed of muscle healing and growth. The easiest way to prevent a deficiency of it is through eating foods rich in protein, as those typically contain necessary amino acids.

Leucine is an amino acid with chemical formula C6H13NO2. It is key for protein synthesis, metabolism, tissue repair, and certain tasks that are also done by Isoleucine. It is known to activate mTOR cell signalng pathways, which stimulate protein synthesis and prevent breakdown of protein. It is found bin animal protein, plant-based protein, and nuts.

D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using CAD.

Condition

Column Primesep 100, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 5/95%
Buffer AmFM pH 3.0 – 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds D-Isoleucine, D-Leucine, DL-Isoleucine, L-Alloisoleucine, L-Isoleucine, allo-D-isoleucine, Isoleucine, Leucine

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
D-Isoleucine
D-Leucine
DL-Isoleucine
Isoleucine
L-Alloisoleucine
L-Isoleucine
Leucine
allo-D-isoleucine

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of Essential Amino Acids on Primesep 100 Column

March 11, 2019

HPLC Method for Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, L-Isoleucine, D-Leucine, Phenylalanine, Histidine, L-Histidine hydrochloride monohydrate, Tryptophan, Amino Acids, L-Threonine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, L-Isoleucine, D-Leucine, Phenylalanine, Histidine, L-Histidine hydrochloride monohydrate, Tryptophan, Amino Acids, L-Threonine.

L-Threonine is an essential amino acid with the chemical formula C4H9NO3.  It cannot be produced within the body and must be obtained through consuming it. It’s found in many protein-rich foods, including but not limited to eggs, meat, dairy, legumes, and seeds. It is necessary in the body as a building block of protein like collagen and elastin. The two proteins are crucial for skin, hair, and connective issue.

L-Valine is an essential amino acid with the chemical formula C5H11NO2. It cannot be produced within the body and must be obtained through consuming it. It’s found in foods including but not limited to nuts, legumes, whole grains, and seeds. It is especially beneficial for athletes.  It is important for muscle repair,  growth, and energy regulation.

DL-Methionine is an essential amino acid with the chemical formula C5H11NO2S.  It cannot be produced within the body and must be obtained through consuming it. It is required for protein synthesis. It also helps build and repair tissue including, but not limited to, skin, hair, muscles, and nails. In a veterinary context, DL-Methionine is used to address bladder issues in dogs.

L-Isoleucine is an essential amino acid with the chemical formula C6H13NO2.  It cannot be produced within the body and must be obtained through consuming it. It is a building block of protein that are essential for muscle growth, repair, and other bodily functions.  It also helps regulate blood sugar levels and supports the immune system. It is found in foods like meat, fish, eggs, dairy, beans, lentils, nuts, and seeds.

L-Leucine is an essential amino acid with the chemical formula C6H13NO2. It cannot be produced within the body and must be obtained through consuming it. It stimulates production of protein that are essential for muscle building and repair. Meats are the easiest way to get L-Leucine in significant amounts.

L-Phenylalanine is an essential amino acid with the chemical formula C6H9NO2. It cannot be produced within the body and must be obtained through consuming it. It is typically found in high protein foods such as meat, eggs, and fish. Outside of being important for creation of protein, it is also used in treatment for skin disorders and depression.

L-Histidine is an essential amino acid with the chemical formula C9H11N3O2. It cannot be produced within the body and must be obtained through consuming it. It s fundamental for repair of damaged tissue, growth of muscles, and making of blood cells. Outside of protein, it also has the unique property of being able to act as a buffer to help maintain stable pH levels in the body. Sources of it include meat, fish, dairy products, beans, and nuts.

L-Tryptophan is an essential amino acid with the chemical formula C11H12N2O2. It cannot be produced within the body and must be obtained through consuming it. Like the other essential proteins, it is a building block for protein and muscle tissue, but it is also converted in the body into serotonin, which affects mood. L-Tryptophan is also used in treatments for severe PMS symptoms, depression, and insomnia. It is naturally found in red meat, poultry eggs, and dairy.

Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, L-Isoleucine, D-Leucine, Phenylalanine, Histidine, L-Histidine hydrochloride monohydrate, Tryptophan, Amino Acids, L-Threonine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition

Column Primesep 100, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 25/75%
Buffer Gradient H2SO4 0.05-0.2% 25 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Valine, D-Valine, Methionine, L-Methionine, Isoleucine, D-Isoleucine, DL-Isoleucine, L-Isoleucine, D-Leucine, Phenylalanine, Histidine, L-Histidine hydrochloride monohydrate, Tryptophan, Amino Acids, L-Threonine

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Amino Acids
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Histidine hydrochloride monohydrate
L-Isoleucine
L-Methionine
L-Threonine
Methionine
Phenylalanine
Tryptophan
Valine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of allo-D-isoleucine and L- Isoleucine on Primesep 100

March 20, 2018

HPLC Method for Isoleucine, L-Isoleucine, allo-D-isoleucine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Isoleucine, L-Isoleucine, allo-D-isoleucine 

Allo-D-isoleucine is a non-proteinogenic amino acid with C6H13NO molecular formula. It is most commonly used in protein synthesis, neuroprotection, and immune modulation. It is also crucial in synthesis of peptides and can support muscle growth and recovery.

L- Isoleucine is a branched-chain amino acid with C6H13NO2 molecular formula. During chemical reactions, it serves as a nitrogen donor. It is also involved in glucose consumption, fatty acid metabolism, and immune functions. It can be found in a variety of animal and plant products, including meat, eggs, dairy, soybeans, lentils, peas, nuts, and seeds. Healthcare providers may encourage athletes and people with muscle atrophy to take it as a supplement.  Excessive consumption of it may lead to side effects including nausea, vomiting, and diarrhea.

Isoleucine, L-Isoleucine, allo-D-isoleucine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a trifluoroacetic acid buffer. Detection is performed using CAD.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA, FA
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Allo-Isoleucine, Isoleucine, Leucine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Isoleucine
L-Isoleucine
allo-D-isoleucine

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of allo-D-isoleucine and L- Isoleucine on Primesep 200

March 20, 2018

HPLC Method for L-Isoleucine, allo-D-isoleucine on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Isoleucine, allo-D-isoleucine 

Allo-D-isoleucine is a non-proteinogenic amino acid with C6H13NO molecular formula. It is most commonly used in protein synthesis, neuroprotection, and immune modulation. It is also crucial in synthesis of peptides and can support muscle growth and recovery.

L- Isoleucine is a branched-chain amino acid with C6H13NO2 molecular formula. During chemical reactions, it serves as a nitrogen donor. It is also involved in glucose consumption, fatty acid metabolism, and immune functions. It can be found in a variety of animal and plant products, including meat, eggs, dairy, soybeans, lentils, peas, nuts, and seeds. Healthcare providers may encourage athletes and people with muscle atrophy to take it as a supplement.  Excessive consumption of it may lead to side effects including nausea, vomiting, and diarrhea.

L-Isoleucine, allo-D-isoleucine can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a trifluoroacetic acid buffer. Detection is performed using CAD.

ColumnPrimesep 200, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O
BufferTFA, FA
Flow Rate1.0 ml/min
DetectionCAD (Corona) MS- compatible mobile phase
Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing CompoundsAllo-Isoleucine, Isoleucine, Leucine

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
L-Isoleucine
allo-D-isoleucine

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.