Parabens

Parabens

Synonyms

    Applications:

    HPLC Analysis of Paraben Preservatives on Newcrom AH Column

    2020-10-29
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    Condition

    Column Newcrom AH, 3.2x100 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O - 30/70%
    Buffer H2SO4 - 0.1%
    Flow Rate 0.5 ml/min
    Detection UV, 270 nm
     

    Description

    Class of Compounds Preservatives,  Hydrophobic
    Analyzing Compounds Methyl Paraben, Ethyl Paraben, Propyl Paraben
     
    Application Analytes:
    Ethylparaben
    Methylparaben
    Methylparaben sodium
    Parabens
    Propylparaben
    Propylparaben sodium

    HPLC Separation of Parabens and Benzoic Acid

    2006-06-05
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    Condition 1

    Column Newcrom B, 3.2x100 mm, 3 µm, 100A
    Mobile Phase MeCN/H2O - 40/60%
    Buffer
    Flow Rate 0.5 ml/min
    Detection UV 275nm
     
    Parabens possess antibacterial and antifungal properties and are therefore widely used in pharmaceutical and cosmetic industries as preservatives in products. Parabens and benzoic acid can be baseline separated in a short time frame using Primesep B2 reverse-phase HPLC column with a simple mobile phase of water, acetonitrile (ACN, MeCN) and phosphoric acid of 0.1% as buffer. UV detection at 210nm.

    Condition 2

    Column Primesep B2, 4.6x150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer H3PO4
    Flow Rate 1.0 ml/min
    Detection UV, 210 nm
     

    Description

    Class of Compounds Acid, Hydrophilic, Preservative
    Analyzing Compounds Methyl paraben, Benzoic Acid, Propyl paraben
     
    Application Analytes:
    Benzoic Acid
    Methylparaben
    Parabens
    Propylparaben

    Application Detection:
    UV Detection