HPLC Separation of Parabens and Benzoic Acid

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Condition 1

Column Newcrom B, 3.2x100 mm, 3 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Flow Rate 0.5 ml/min
Detection UV 275nm
Parabens possess antibacterial and antifungal properties and are therefore widely used in pharmaceutical and cosmetic industries as preservatives in products. Parabens and benzoic acid can be baseline separated in a short time frame using Primesep B2 reverse-phase HPLC column with a simple mobile phase of water, acetonitrile (ACN, MeCN) and phosphoric acid of 0.1% as buffer. UV detection at 210nm.

Condition 2

Column Primesep B2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds Acid, Hydrophilic, Preservative
Analyzing Compounds Methyl paraben, Benzoic Acid, Propyl paraben
Application Analytes:
Benzoic Acid