CAS Number | 54-16-0 |
---|---|
Molecular Formula | C10H9NO3 |
Molecular Weight | 191.186 |
InChI Key | DUUGKQCEGZLZNO-UHFFFAOYSA-N |
LogP | 1.1 |
Synonyms |
|
Column | Newcrom B , 3.2×50 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 20/80% |
Buffer | H3PO4 – 0.1% |
Flow Rate | 0.5 ml/min |
Detection | UV, 275 nm |
Vanilmandelic, hydroxyindoleacetic and homovanillic acids are metabolites of various neurotransmitters that are used in urine tests to measure the levels of those neurotransmitters in the body. They can be baseline separated on a reverse-phase Primesep D HPLC column by varying the buffers and pH of the mobile phase consisting of water and acetonitrile (MeCN, ACN). UV detection at 230nm.
Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | TFA, AmFm |
Flow Rate | 1.0 ml/min |
Detection | UV, 230 nm |
Class of Compounds |
Acid, Monocarboxylic acid, Hydrophilic, Ionizable |
Analyzing Compounds | Vanilmandelic, Hydroxyindoleacetic, Homovanillic Acid |
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
Select optionsThe Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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