5-Hydroxyindole-3-Acetic Acid

5-Hydroxyindole-3-Acetic Acid

CAS Number54-16-0
Molecular FormulaC10H9NO3
Molecular Weight191.186
  • 5-Hydroxyindole-3-acetic acid
  • 54-16-0
  • 5-HIAA
  • 2-(5-hydroxy-1H-indol-3-yl)acetic acid
  • 5-Hydroxyindoleacetic acid
  • 5-Hydroxyheteroauxin
  • (5-Hydroxy-1H-indol-3-yl)acetic acid
  • 5-Oxyindoleacetic acid
  • 5-Hydroxy-IAA
  • 5-Hydroxyindol-3-ylacetic acid
  • Hydroxyindoleacetic acid
  • 5-Hydroxy-1H-indole-3-acetic acid
  • 5-Hydroxyindolacetic acid
  • 5-Hydroxy-3-indoleacetic acid
  • 5-Hydroxyindolylacetic acid
  • 1H-Indole-3-acetic acid, 5-hydroxy-
  • 1321-73-9
  • Indole-3-acetic acid, 5-hydroxy-
  • 5-Hydroxyindoleacetate
  • NSC 90432
  • CCRIS 4423
  • 1H-Indoleacetic acid, hydroxy-
  • 5-hydroxy-indole-3-acetic acid
  • EINECS 200-195-4
  • BRN 0168797
  • 5-Hydroxyindol-3-acetic acid
  • CHEBI:27823
  • 2-(5-hydroxyindol-3-yl)acetic acid


HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids


View on hplc.cloud

Condition 1

Column Newcrom B , 3.2×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4 – 0.1%
Flow Rate 0.5 ml/min
Detection UV, 275 nm


Vanilmandelic, hydroxyindoleacetic and homovanillic acids are metabolites of various neurotransmitters that are used in urine tests to measure the levels of those neurotransmitters in the body. They can be baseline separated on a reverse-phase Primesep D HPLC column by varying the buffers and pH of the mobile phase consisting of water and acetonitrile (MeCN, ACN). UV detection at 230nm.

Condition 2

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TFA,  AmFm
Flow Rate 1.0 ml/min
Detection UV, 230 nm



Class of Compounds
 Acid, Monocarboxylic acid,  Hydrophilic, Ionizable
Analyzing Compounds Vanilmandelic, Hydroxyindoleacetic, Homovanillic Acid


Application Column

Newcrom B

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.

Select options

Primesep D

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
5-Hydroxyindole-3-Acetic Acid
Homovanillic Acid
Vanilmandelic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.