| CAS Number | 55-10-7 |
|---|---|
| Molecular Formula | C9H10O5 |
| Molecular Weight | 198.174 |
| InChI Key | CGQCWMIAEPEHNQ-UHFFFAOYSA-N |
| LogP | -0.2 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Vanilmandelic Acid
June 9, 2026
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If you are looking for optimized HPLC method to analyze Vanilmandelic Acid check our HPLC Applications library
UV/Vis.: λmax: 222, 237, 280 nm
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in ethanol. This analysis was done with the mobile phase of hexane, IPA, and TFA.
HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids
November 21, 2006
| Column | Newcrom B , 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 275 nm |
Vanilmandelic, hydroxyindoleacetic and homovanillic acids are metabolites of various neurotransmitters that are used in urine tests to measure the levels of those neurotransmitters in the body. They can be baseline separated on a reverse-phase Primesep D HPLC column by varying the buffers and pH of the mobile phase consisting of water and acetonitrile (MeCN, ACN). UV detection at 230nm.
| Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | TFA, AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Acid, Monocarboxylic acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Vanilmandelic, Hydroxyindoleacetic, Homovanillic Acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsHomovanillic Acid
Vanilmandelic Acid
