HPLC Chiral Separation of Vanillylmandelic Acid on Cyralis CM Column

HPLC Method for Analysis of Vanillylmandelic Acid on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Vanillylmandelic Acid.

Vanillylmandelic acid is a chemical intermediate with the chemical formula C9H10O5. It is primarily used primarily in synthesis of artificial vanilla flavoring. It is also an end-stage metabolite of epinephrine and norepinephrine. You can find detailed UV spectra of Vanillylmandelic Acid and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Vanillylmandelic Acid enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis. Additional peaks that are visible on the chromatogram come from impurities and additives.

Vanillylmandelic Acid can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA/TFA- 70/28.5/1.5%
Flow Rate1.0 mL/min
DetectionUV 280 nm
Resolution2.51

Description

Class of CompoundsIntermediate
Analyzing CompoundsVanillylmandelic Acid

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Vanillylmandelic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.