CAS Number | 50-67-9 |
---|---|
Molecular Formula | C10H12N2O |
Molecular Weight | 176.219 |
InChI Key | QZAYGJVTTNCVMB-UHFFFAOYSA-N |
LogP | 0.2 |
Synonyms |
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Applications:
HPLC Method For Analysis Of Dopamine and Serotonin on Primesep 200 Column
March 25, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Dopamine and Serotonin
Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. Serotonin is another key neurotransmitter that has pharmaceutical applications – namely as the key ingredient in antidepressant and anti-anxiety medications.These two neurotransmitters can be retained, separated, and analyzed on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be UV detected at 280 nm with high resolution and peak symmetry.
Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 20/80% |
Buffer | Ammonium Formate pH 3.0 – 40 mM |
Flow Rate | 1.0 ml/min |
Detection | UV, 280 nm, |
Class of Compounds |
Neurotransmitters |
Analyzing Compounds | Dopamine, Serotonin |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSerotonin
HPLC Separation of Biogenic Amines on Primesep 100 Column
April 30, 2020
HPLC Method for Tryptophan, Serotonin, N,N-Dimethyltryptamine, Tryptamine, N,N-Dimethyl-5-methoxytryptamine, N,N-dimethyltryptophan on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tryptophan, Serotonin, N,N-Dimethyltryptamine, Tryptamine, N,N-Dimethyl-5-methoxytryptamine, N,N-dimethyltryptophan.
Tryptophan is an essential amino acid used in the synthesis of proteins. It cannot be produced by the body and therefore must be consumed through a diet of meats, eggs, dairy, seeds, and nuts. The proteins that it helps synthesize are important for muscle growth and repair. Tryptophan is also converted into serotonin, which is responsible for mood, appetite, and pain reception. It is further down converted into melatonin. Tryptophan is also used in treatments for severe PMS symptoms, depression, and insomnia. It is naturally found in red meat, poultry eggs, and dairy. It’s chemical formula is C11H12N2O2. You can find detailed UV spectra of Tryptophan and information about its various lambda maxima by visiting the following link.
Tryptamine is an indolamine derived from tryptophan with the chemical formula C10H12N2. It is naturally found in plants, fungi, and animals. It helps with regulating the activity of dopaminergic, serotonergic, and glutamatergic systems. You can find detailed UV spectra of Tryptamine and information about its various lambda maxima by visiting the following link.
Serotonin is a neurotransmitter derived from tryptophan with the chemical formula C₁₀H₁₂N₂O. Serotonin is a chemical messenger that is crucial for bodily functions. It helps regulate mood and reduce anxiety, digestion, blood clotting, and contributes to the sleep-wake cycle. Low serotonin levels are often associated with a variety of mental illnesses including but not limited to Obsessive Compulsive Disorder (OCD), Post-Traumatic Stress Disorder (PTSD), Depression, and Anxiety.
Dimethyltryptamine is a psychedelic substance derived from tryptamine with the chemical formula C12H16N2. It works as an agonist for Serotonin. It is not approved for medical use. Due to it’s high risk of abuse, it is a Schedule I drug in the United States.
5-MeO-DMT, also known as 5-methoxy-N,N-dimethyltryptamine), O-methylbufotenin, and mebufotenin, is a psychedelic substances derived from tryptamine found in plants and toad secretions. Between 1970s to 1990s, a certain religious sect believed the use of mebufotenin to be sacred to their religious practice. It is currently considered a Schedule I controlled substance in the United States, however.
All the compounds have similar structures and can present difficulties to separation in reverse-phase HPLC. They can be separated using the Primesep 100 mixed-mode column in gradient analysis with acetonitrile (ACN) and water mobile phase with ammonium formate (AmFm) buffer, making the method MS-compatible. The amines can also be UV detected at 280nm.
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | Gradient MeCN – 40-50%, 15 min |
Buffer | Gradient AmFm pH 3.0- 20-80 mM, 15 min |
Flow Rate | 1.0 ml/min |
Detection | UV 280 nm, MS-compatible mobile phase |
Class of Compounds |
Drug, Hydrophilic, Supplements, Monoamine, Neurotransmitter |
Analyzing Compounds | Tryptophan, Serotonin, N,N-Dimethyltryptamine, Tryptamine, N,N-Dimethyl-5-methoxytryptamine, N,N-dimethyltryptophan |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
N,N-Dimethyltryptamine
N,N-dimethyltryptophan
Serotonin
Tryptamine
Tryptophan
HPLC Determination of Serotonin on Primesep 200 Column
April 6, 2020
HPLC Method for Serotonin on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Serotonin.
Serotonin is a neurotransmitter derived from tryptophan with the chemical formula C₁₀H₁₂N₂O. Serotonin is a chemical messenger that is crucial for bodily functions. It helps regulate mood and reduce anxiety, digestion, blood clotting, and contributes to the sleep-wake cycle. Low serotonin levels are often associated with a variety of mental illnesses including but not limited to Obsessive Compulsive Disorder (OCD), Post-Traumatic Stress Disorder (PTSD), Depression, and Anxiety.
Serotonin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 30/70% |
Buffer | AmFm pH 3.0- 20 mM |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm, MS-compatible mobile phase |
Class of Compounds |
Drug, Hydrophilic, Supplements, Monoamine, Neurotransmitter |
Analyzing Compounds | Serotonin |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Separation of Neurotransmitters
November 25, 2011
The neurotransmitters dl-DOPA, creatinine, hydroxytryptophan (5-HTP), and serotonin are separated in less than 4 minutes on a short 50 mm Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Ion-pair reagents were not needed for retention of these polar, hydrophilic compounds, instead, a combination of ion-exchange and reversed-phase interactions were used.
Column | Primesep 200, 4.6×50 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 80/20% |
Buffer | TFA – 0.05% |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Hormone |
Analyzing Compounds | dl-DOPA, Creatinine, Hydroxytryptophan (5-HTP), Serotonin |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsDOPA (3,4-dihydroxy-L-phenylalanine)
Hydroxytryptophan
Serotonin
Separation of Serotonin, Dopamine, and Related Compounds
August 22, 2008
Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of the catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. The method is very sensitive to amount of ACN, buffer and buffer pH. The retention time changes with variation of the main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by a combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.).
Column | Obelisc N, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O |
Buffer | AmAc pH 4.0- 30 mM |
Flow Rate | 1.0 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
Analyzing Compounds | Homovanillic acid, Dihydroxyphenyl acetic acid, Serotonin, Dopamine, Epinephrine, Hydroxytryptophan, DOPA |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsDOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Epinephrine
Homovanillic Acid
Hydroxytryptophan
Norepinephrine
Serotonin
HPLC Separation of Neurotransmitters on Primesep P
June 5, 2004

Dopamine, norphenylephrine and serotonin are separated on Primesep P HPLC column. Base line separation of these neurotransmitters can be achieved using mix-mode approach. Primesep P provides retention by reverse phase, pi-pi and cation-exchange mechanisms. Method can be used for fast quantitation of these compounds in various sample matrices. Detection technique ranges from UV to ELSD, LC/MS, RI and CAD. Primesep P column can be used with tetrahydrofuran or with 100% aqueous mobile phase to enhance pi-pi interaction.
Application Column
Primesep P
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Norphenylephrine
Serotonin