Serotonin

CAS Number50-67-9
Molecular FormulaC10H12N2O
Molecular Weight176.219
InChI Key QZAYGJVTTNCVMB-UHFFFAOYSA-N
LogP0.2
Synonyms
  • serotonin
  • 5-HYDROXYTRYPTAMINE
  • 3-(2-Aminoethyl)-1H-indol-5-ol
  • Enteramine
  • 5-HT
  • Antemovis
  • Serotonine
  • Thrombocytin
  • Thrombotonin
  • Ds substance
  • Hippophain
  • Antemoqua
  • Substance DS
  • Substanz DS
  • 1H-Indol-5-ol, 3-(2-aminoethyl)-
  • 5-Hta
  • Tryptamine, 5-hydroxy-
  • 3-(2-Aminoethyl)indol-5-ol
  • Enteramin
  • UNII-333DO1RDJY
  • Indol-5-ol, 3-(2-aminoethyl)-
  • 3-(beta-Aminoethyl)-5-hydroxyindole
  • 5-Hydroxy-3-(beta-aminoethyl)indole
  • EINECS 200-058-9
  • 3-(2-Amino-ethyl)-1H-indol-5-ol
  • BRN 0143524
  • CHEBI:28790
  • QZAYGJVTTNCVMB-UHFFFAOYSA-N
  • 1H-Indol-5-ol,3-(2-aminoethyl)-
  • Hippophaine
  • H-8000
  • 5-Hydroxytriptamine
  • 5-Hydroxyltryptamine
  • 5-hydroxy-tryptamine
  • 5-hydroxyl tryptamine
  • GTPL5
  • 5-22-12-00016 (Beilstein Handbook Reference)
  • Serotonin, analytical standard
  • SPBio_002262
  • 5-Hydroxytryptamine, free base
  • 3-(2-Aminoethyl)-1H-indol-5-ol
  • #MCULE-8788459983
  • 3-(.beta.-Aminoethyl)-5-hydroxyindole
  • 5-Hydroxy-3-(.beta.-aminoethyl)indole
  • 5-Hydroxytryptamine, 5-HT, Enteramine
  • Thrombocytin, 3-(|A-Aminoethyl)-5-hydroxyindole, Thrombotonin
  • 2-imino-1-methylimidazolidin-4-one; 3-(2-aminoethyl)-1H-indol-5-ol; bis(sulfuric acid)

Applications:

HPLC Method For Analysis Of Dopamine and Serotonin on Primesep 200 Column

2022-03-25

Separation type: Liquid Chromatography Mixed-mode High Performance Liquid Chromatography (HPLC) Method for Analysis of Dopamine and Serotonin Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. Serotonin is another key neurotransmitter that has pharmaceutical applications - namely as the key ingredient in antidepressant and anti-anxiety medications.These two neurotransmitters can be retained, separated, and analyzed on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be UV detected at 280 nm with high resolution and peak symmetry.  

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Ammonium Formate pH 3.0 - 40 mM
Flow Rate 1.0 ml/min
Detection UV, 280 nm,
 

Description

Class of Compounds Neurotransmitters
Analyzing Compounds  Dopamine, Serotonin
 
Application Analytes:
Dopamine
Serotonin

HPLC Separation of Biogenic Amines on Primesep 100 Column

2020-04-30
Tryptophan is an essential amino acid used in the synthesis of proteins. Tryptamine is structurally similar to tryptophan. Serotonin is a neurotransmitter derived from tryptophan. Dimethyltryptamine and 5-MeO-DMT are psychedelic substances derived from tryptamine. All the compounds have similar structures and can present difficulties to separation in reverse-phase HPLC. They can be separated using the Primesep 100 mixed-mode column in gradient analysis with acetonitrile (ACN) and water mobile phase with ammonium formate (AmFm) buffer, making the method MS-compatible. The amines can also be UV detected at 280nm.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 40-50%, 15 min
Buffer Gradient AmFm pH 3.0- 20-80 mM, 15 min
Flow Rate 1.0 ml/min
Detection UV 280 nm,  MS-compatible mobile phase
 

Description

Class of Compounds Drug, Hydrophilic, Supplements, Monoamine,  Neurotransmitter
Analyzing Compounds Serotonin, Tryptophan, N,N-dimethyltryptophan, N,N-Dimethyl-5-methoxytryptamine, Tryptamine, N,N-dimethyltryptamine
 
Application Analytes:
N,N-Dimethyl-5-methoxytryptamine
N,N-Dimethyltryptamine
N,N-dimethyltryptophan
Serotonin
Tryptamine
Tryptophan

HPLC Determination of Serotonin on Primesep 200 Column

2020-04-06
HPLC.cloud View on hplc.cloud HPLC Determination of Serotonin on Primesep 200 Column_1241

Condition

Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV 275 nm,  MS-compatible mobile phase
 

Description

Class of Compounds Drug, Hydrophilic, Supplements, Monoamine,  Neurotransmitter
Analyzing Compounds Serotonin
 
Application Analytes:
Serotonin

HPLC Separation of Neurotransmitters

2011-11-25
The neurotransmitters dl-DOPA, creatinine, hydroxytryptophan (5-HTP), and serotonin are separated in less than 4 minutes on a short 50 mm Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Ion-pair reagents were not needed for retention of these polar, hydrophilic compounds, instead, a combination of ion-exchange and reversed-phase interactions were used.

Condition

Column Primesep 200, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 80/20%
Buffer TFA - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds dl-DOPA, Creatinine, Hydroxytryptophan (5-HTP), Serotonin
 
Application Analytes:
Creatinine
DOPA (3,4-dihydroxy-L-phenylalanine)
Hydroxytryptophan
Serotonin

Application Detection:
UV Detection

Separation of Serotonin, Dopamine, and Related Compounds

2008-08-22
Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of the catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. The method is very sensitive to amount of ACN, buffer and buffer pH. The retention time changes with variation of the main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by a combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.).

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Homovanillic acid, Dihydroxyphenyl acetic acid, Serotonin, Dopamine, Epinephrine, Hydroxytryptophan, DOPA
 
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Epinephrine
Homovanillic Acid
Hydroxytryptophan
Norepinephrine
Serotonin

Application Detection:
UV Detection