Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Dopamine and Serotonin
Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. Serotonin is another key neurotransmitter that has pharmaceutical applications – namely as the key ingredient in antidepressant and anti-anxiety medications.These two neurotransmitters can be retained, separated, and analyzed on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be UV detected at 280 nm with high resolution and peak symmetry.
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm, |
| Class of Compounds |
Neurotransmitters |
| Analyzing Compounds | Dopamine, Serotonin |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSerotonin
