HPLC Method for Dopamine, Serotonin on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Dopamine, Serotonin.
Dopamine is a neurotransmitter with the chemical formula C8H11NO2. It is released during pleasurable activities, reinforcing those behaviors, and motivating people to continue seeking them out. Dopamine influences the release of other hormones. A deficiency of it can influence movement and lead to conditions like Parkinson’s disease. You can find detailed UV spectra of Dopamine and information about its various lambda maxima by visiting the following link.
Serotonin is a neurotransmitter derived from tryptophan with the chemical formula C₁₀H₁₂N₂O. Serotonin is a chemical messenger that is crucial for bodily functions. It helps regulate mood and reduce anxiety, digestion, blood clotting, and contributes to the sleep-wake cycle. Low serotonin levels are often associated with a variety of mental illnesses including but not limited to Obsessive Compulsive Disorder (OCD), Post-Traumatic Stress Disorder (PTSD), Depression, and Anxiety. You can find detailed UV spectra of Serotonin and information about its various lambda maxima by visiting the following link.
Dopamine, Serotonin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm, |
| Class of Compounds |
Neurotransmitters |
| Analyzing Compounds | Dopamine, Serotonin |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Serotonin
