N,N-Dimethyltryptamine

N,N-Dimethyltryptamine structural formula

CAS Number61-50-7
Molecular FormulaC12H16N2
Molecular Weight188.275
InChI KeyDMULVCHRPCFFGV-UHFFFAOYSA-N
LogP2.00
Synonyms
  • N,N-Dimethyltryptamine
  • 2-(1H-Indol-3-yl)-N,N-dimethylethan-1-amine
  • 1H-Indole-3-ethanamine, N,N-dimethyl-
  • 61-50-7
  • 2-(1H-Indol-3-yl)-N,N-dimethylethanamine
  • 2-(3-Indolyl)ethyldimethylamine
  • NSC 63795
  • BRN 0138259
  • EINECS 200-508-4
  • UNII-WUB601BHAA
  • (2-Indol-3-ylethyl)dimethylamine
  • (psychogenic)
  • 2-(1H-Indol-3-yl)-N,N-dimethylethanamine (ACD/Name 4.0)
  • 3-(2-(Dimethylamino)ethyl)-Indole
  • 3-(2-dimethylaminoethyl)indole
  • 3-[2- (Dimethylamino)ethyl]-Indole
  • 3-[2-(dimethylamino)ethyl]indole
  • DMT
  • Dimethyltryptamine(DMT)
  • Indolalkylamine der
  • N,N-Dimethyl-1H-Indole-3-ethanamine
  • N,N-Dimethyl-1H-Indole-3-ethanamine (9CI)
  • N,N-dimethyl-1H-indole-3-ethylamine
  • N-(2-(1H-Indol-3-yl)ethyl)-N,N-dimethylamine (ACD/Name 4.0)
  • [2-(1H-indol-3-yl)ethyl]dimethylamine
  • 68677-25-8

Applications:

HPLC Separation of Biogenic Amines on Primesep 100 Column

April 30, 2020


Tryptophan is an essential amino acid used in the synthesis of proteins. Tryptamine is structurally similar to tryptophan. Serotonin is a neurotransmitter derived from tryptophan. Dimethyltryptamine and 5-MeO-DMT are psychedelic substances derived from tryptamine. All the compounds have similar structures and can present difficulties to separation in reverse-phase HPLC. They can be separated using the Primesep 100 mixed-mode column in gradient analysis with acetonitrile (ACN) and water mobile phase with ammonium formate (AmFm) buffer, making the method MS-compatible. The amines can also be UV detected at 280nm.

Condition
Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 40-50%, 15 min
Buffer Gradient AmFm pH 3.0- 20-80 mM, 15 min
Flow Rate 1.0 ml/min
Detection UV 280 nm,  MS-compatible mobile phase

 

Description
Class of Compounds
 Drug, Hydrophilic, Supplements, Monoamine,  Neurotransmitter
Analyzing Compounds Serotonin, Tryptophan, N,N-dimethyltryptophan, N,N-Dimethyl-5-methoxytryptamine, Tryptamine, N,N-dimethyltryptamine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
N,N-Dimethyl-5-methoxytryptamine
N,N-Dimethyltryptamine
N,N-dimethyltryptophan
Serotonin
Tryptamine
Tryptophan
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of N,N-Dimethyltryptamine on Newcrom R1 HPLC column

May 16, 2018
Separation of N,N-Dimethyltryptamine on Newcrom R1 HPLC column

N,N-Dimethyltryptamine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
N,N-Dimethyltryptamine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.