Separation of Benzoic and Acetylbenzoic acid in Hydrogen-Bonding Mode

A general approach for analysis of various organic acids with MS detection in negative mode was developed using hydrogen-bonding stationary phase - SHARC 1. A highly sensitive method allows to analyze traces of organic acids in various matrices using ACN/MeOH/ammonia mobile phase. In hydrogen-bonding chromatography acetonitrile is a weaker solvent and alcohol is a stronger solvent. Various gradient and isocratic conditions can be used


Column Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH - 95/5%
Buffer Ammonia 10mM
Flow Rate 005 ml/min
Detection UV, 250 nm


Class of Compounds  Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Benzoic Acid, Acetylbenzoic acid
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Application Analytes:
4-Acetylbenzoic Acid
Benzoic Acid

Application Detection:
UV Detection
ELSD/MS Detection