Separation of Benzoic and Acetylbenzoic acid in Hydrogen-Bonding Mode

A general approach for analysis of various organic acids with MS detection in negative mode was developed using hydrogen-bonding stationary phase – SHARC 1. A highly sensitive method allows to analyze traces of organic acids in various matrices using ACN/MeOH/ammonia mobile phase. In hydrogen-bonding chromatography acetonitrile is a weaker solvent and alcohol is a stronger solvent. Various gradient and isocratic conditions can be used


Column Sharc 1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH – 95/5%
Buffer Ammonia 10mM
Flow Rate 005 ml/min
Detection UV, 250 nm



Class of Compounds
 Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Benzoic Acid, Acetylbenzoic acid


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
4-Acetylbenzoic Acid
Benzoic Acid

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.