HPLC Separation of Acedoben and Inosine on SHARC 1 Column

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Inosine pranobex is an antiviral drug. A combination of inosine and dimepranol acedoben (a salt of acetamidobenzoic acid and dimethylaminoisopropanol) has no effect on viral particles itself. Instead, it acts as an immunostimulant. It is most commonly used to treat the rare measles complication subacute sclerosing panencephalitis in conjunction with intrathecal interferon therapy.
Chromatography of these two compounds can be difficult due to their high polarity. But both compounds can be well retained and separated using anhydrous (water-free) conditions using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase with volatile buffer containing Formic Acid 0.1% and AmFm – 0.01%, making the method MS-compatible. Both compounds can also be UV detected at 270 nm.


Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer Formic Acid 0.1% , AmFm – 0.01%
Flow Rate 1.0 ml/min
Detection UV 270 nm



Class of Compounds
Nucleoside monophosphate, Hydrophilic, Ionizable
Analyzing Compounds Inosine, Acedoben


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
4-Aminobenzoic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.