HPLC Separation of Inosine and Deoxyinosine

Condition
Column Sharc 1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH – 97/3%
Buffer Formic Acid 0.1% , AmFm – 0.01%
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description
Class of Compounds
 Nucleoside monophosphate, Hydrophilic, Ionizable
Analyzing Compounds Inosine, Deoxyinosine

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
2-Deoxyinosine
Inosine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.