HPLC Dye Separation on SHARC1 Column



Column Sharc 1, 3.2x100 mm, 3 µm, 100A
Mobile Phase MeCN/EtOH - 99/1%
Buffer H2SO4 - 0.5%
Flow Rate 1.5 ml/min
Detection UV, 500, 650 nm


Class of Compounds Dye, Hydrophilic, Ionizable
Analyzing Compounds Methyl Red, Methyl Orange, Methylene Blue, Nile Blue A
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Application Analytes:
Methyl Orange
Methyl Red
Methylene Blue
Nile Blue A

Application Detection:
UV Detection