HPLC Dye Separation on SHARC1 Column



Column Sharc 1, 3.2×100 mm, 3 µm, 100A
Mobile Phase MeCN/EtOH – 99/1%
Buffer H2SO4 – 0.5%
Flow Rate 1.5 ml/min
Detection UV, 500, 650 nm



Class of Compounds
Dye, Hydrophilic, Ionizable
Analyzing Compounds Methyl Red, Methyl Orange, Methylene Blue, Nile Blue A


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
Methyl Orange
Methyl Red
Methylene Blue
Nile Blue A

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.