Nile Blue A

CAS Number3625-57-8
Molecular FormulaC40H40N6O6S
Molecular Weight732.856
InChI KeyQIRDPEPUXNCOLD-UHFFFAOYSA-N
Synonyms
  • Nile Blue sulfate
  • Nile Blue A
  • Nile Blue A sulfate
  • 3625-57-8
  • Nile blue
  • NSC 3250
  • EINECS 222-832-5
  • NSC 34914
  • MFCD00064529
  • SBB057664

Applications:

HPLC Method for Analysis of Dyes

March 1, 2018

HPLC Separation of Dyes

Alizarin a red pigment that comes as a red powder with a slight orange tint. When dissolved it appears bright red with a slight pink undertone. Alizarin is also known as Mordant Red 11 or Turkey Red. Eosin Y is a pigment that comes as an orange powder that when dissolved in water turns bright orange. Eosin is a fluorescent staining agent. It is used to stain red blood cells, proteins on cytoplasm, collage, and muscle fibers. It is also fluorescence. Fluorescein is a dye that comes in a dark orange powder and is used as a tracer and an indicator. As it says in the name, it is also Fluorescent, obtaining a green-yellow color when under black light. Methyl red an indicator that turns red in acidic solutions (below 4.4 pH), is a shade of orange between 4.5 and 6.1 pH, and turns bright yellow in basic solutions (above pH 6.2). Nile blue, also known as Nile blue A, is used as a stain used in biology and histology as well as a pH indicator. In pH below 7 it becomes lighter shades of glue, and at pH 0 becomes almost completely translucent. At pH of seven it has a dark blue shade, while at pH higher than that it starts becoming more red. Pararosaniline Hydrochloride, also known as Basic Red 9, or C.I. 42500. It is a a magenta solid that is used as a dye. Patent Blue Violet, also known as Patent Blue, is a deep blue pigment that is often used to dye clothes. Resazurin is a blue dye as well as a pH indicator. At pH 3.8 or lower, it is bright orange, but the higher the pH the more blue it becomes, the color stops changing at the pH of 6.5 where it becomes indigo. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain dyes. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
3-Aminoacridine
Alizarin
Carmine
Eosin Y
Fluorescein
Fluorescein
Methyl Red
Nile Blue A
Pararosaniline Hydrochloride
Patent Blue Violet (Patent Blue)
Resazurin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Dye Separation on SHARC1 Column

July 10, 2013

 

Condition

Column Sharc 1, 3.2×100 mm, 3 µm, 100A
Mobile Phase MeCN/EtOH – 99/1%
Buffer H2SO4 – 0.5%
Flow Rate 1.5 ml/min
Detection UV, 500, 650 nm

 

Description

Class of Compounds
Dye, Hydrophilic, Ionizable
Analyzing Compounds Methyl Red, Methyl Orange, Methylene Blue, Nile Blue A

 

Application Column

SHARC 1

The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
Methyl Orange
Methyl Red
Methylene Blue
Nile Blue A

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.