Fluorescein

Fluorescein sodium structural formula

CAS Number518-47-8
Molecular FormulaC20H10Na2O5
Molecular Weight376.275
InChI KeyRGPLGPBQJOQFJS-UHFFFAOYSA-L
LogP2.58
Synonyms
  • Fluorescein sodium
  • Disodium 3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-bis(olate)
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-, sodium salt (1:2)
  • 518-47-8
  • C.I. Acid yellow 73
  • Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-, disodium salt
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-, disodium salt
  • 11824 Yellow
  • 12417 Yellow
  • 2-(3-oxo-6-oxidoxanten-9-il)benzoato de disodio
  • 2-(3-Oxo-6-oxydoxanthen-9-yl)benzoate de disodium
  • 2-(3-oxo-6-oxydoxanthene-9-yl)benzoate de disodium
  • 3058 Uranine
  • 5(6)-Carboxyfluorescein sodium
  • Abbey Uranine
  • ABCO Uranine
  • Acid Yellow 73
  • Aizen Uranine
  • Basacid Yellow 226
  • Basovit Yellow 227
  • C.I. acid yellow 073
  • Calcocid Uranine B 4315
  • Certiqual Fluoresceine
  • Cogilor Yellow 111.10
  • D & C Yellow 8
  • D and C Yellow No. 8
  • D&C Yellow No. 8
  • D&C Yellow No. 8-307225
  • Dinacid Florescence Uranine
  • Dinacid Florescinece Uranine
  • Dinatrium-2-(3-oxo-6-oxidoxanthen-9-yl)benzoat
  • disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
  • Disodium fluorescein
  • Fluo-rectal
  • Fluorescein disodium
  • FLUORESCEIN DISODIUM SALT DYE
  • Fluorescein LT
  • Fluorescein Sodium B.P
  • FLUORESCEIN SODIUM SALT
  • Fluorescein, disodium salt
  • FLUORESCEIN, SODIUM
  • Fluoresceine DTD 842
  • Fluorescite
  • Fluoresein
  • Fluorin
  • Fluor-I-Strip
  • Flurenate
  • Ful-Glo
  • Furanium
  • Hidacid Uranine
  • Neelicol Fluorescein
  • Neelicol Uranine
  • Obiturine
  • Resorcinol phthalein sodium
  • Sicomet Uranin S 45350
  • Sicomet Uranine S 45350
  • Simacid Fluoresceine LT
  • Simacid Fluoresceine Sodium
  • Sodium fluorescein
  • Sodium fluoresceinate
  • Soluble Fluorescein
  • Soluble Fluoresceine BPS
  • SPIRO(ISOBENZOFURAN-1(3H),9'-[9H]XANTHEN]-3-ONE, 3'-6'-DIHYDROXY-, DISODIUM SALTS
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-, sodium salt (1:2)
  • Triacid Fluoresceine
  • Urane U 307027
  • Uranin A
  • Uranin S
  • Uranine
  • Uranine A
  • Uranine A extra
  • Uranine O
  • Uranine SS
  • Uranine SSO
  • Uranine WSS
  • Victacid Uranine
  • Victacid Uranine VDC
  • Calcocid uranine B4315
  • 9-o-Carboxyphenyl-6-hydroxy-3-isoxanthone, disodium salt
  • Disodium 6-hydroxy-3-oxo-9-xanthene-o-benzoate
  • EINECS 208-253-0
  • Fluorescein, soluble
  • Fluor-I-strip A.T.
  • Funduscein
  • NCI-C54706
  • Sodium salt of hydroxy-o-carboxy-phenyl-fluorone
  • Soluble fluoresceine
  • Uranine Yellow
  • Japan Yellow 202(1)
  • Funduscein-25
  • Ak-Fluor
  • UNII-93X55PE38X
  • 121511-05-5
  • 1279055-25-2
  • 135354-40-4
  • 208117-76-4
  • 31949-37-8
  • 3266-99-7
  • 34517-88-9
  • 41935-48-2
  • 574737-69-2
  • 893427-14-0

Applications:

Separation of Fluorescein sodium on Newcrom R1 HPLC column

May 16, 2018
Separation of Fluorescein sodium on Newcrom R1 HPLC column

Fluorescein sodium can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Fluorescein
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.

HPLC Method for Analysis of Dyes

March 1, 2018

HPLC Separation of Dyes

Alizarin a red pigment that comes as a red powder with a slight orange tint. When dissolved it appears bright red with a slight pink undertone. Alizarin is also known as Mordant Red 11 or Turkey Red. Eosin Y is a pigment that comes as an orange powder that when dissolved in water turns bright orange. Eosin is a fluorescent staining agent. It is used to stain red blood cells, proteins on cytoplasm, collage, and muscle fibers. It is also fluorescence. Fluorescein is a dye that comes in a dark orange powder and is used as a tracer and an indicator. As it says in the name, it is also Fluorescent, obtaining a green-yellow color when under black light. Methyl red an indicator that turns red in acidic solutions (below 4.4 pH), is a shade of orange between 4.5 and 6.1 pH, and turns bright yellow in basic solutions (above pH 6.2). Nile blue, also known as Nile blue A, is used as a stain used in biology and histology as well as a pH indicator. In pH below 7 it becomes lighter shades of glue, and at pH 0 becomes almost completely translucent. At pH of seven it has a dark blue shade, while at pH higher than that it starts becoming more red. Pararosaniline Hydrochloride, also known as Basic Red 9, or C.I. 42500. It is a a magenta solid that is used as a dye. Patent Blue Violet, also known as Patent Blue, is a deep blue pigment that is often used to dye clothes. Resazurin is a blue dye as well as a pH indicator. At pH 3.8 or lower, it is bright orange, but the higher the pH the more blue it becomes, the color stops changing at the pH of 6.5 where it becomes indigo. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain dyes. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
3-Aminoacridine
Alizarin
Carmine
Eosin Y
Fluorescein
Fluorescein
Methyl Red
Nile Blue A
Pararosaniline Hydrochloride
Patent Blue Violet (Patent Blue)
Resazurin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.