HPLC Determination of Piracetam on SHARC 1 Column

Separation type: Liquid Chromatography HILIC

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Piracetam

Piracetam, part of the racetam family of drugs, has been marketed as a treatment for myoclonus and as a way to enhance cognition, although neither claim is approved by the FDA.
This racetam can be detected in the low UV regime. Using a SHARC 1 hydrogen-bonding column and a mobile phase consisting of acetonitrile (MeCN) and water without a buffer, Piracetam can be retained, measured, and analyzed. This method can be UV detected at 200 nm with very high resolution


Column SHARC 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O -98/2%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 200 nm



Class of Compounds
 Drug, Racetam
Analyzing Compounds Piracetam


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.