HPLC Analysis of Ethephon in Hydrogen-Bonding Mode

Ethephon is an important compound for the agricultural industry. It can be corrosive, but metabolizes to ethylene quickly in plants. Ethephon was retained on SHARC 1 HILIC/anion-exclusion column. Retention was controlled by adjusting methanol in mobile phase to suppress hydrogen bonding.


Column Sharc 1, 3.2×100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH – 90/10% to 50/50, 6 min
Buffer Ammonia – 30 mM
Flow Rate 1.0 ml/min
Detection ELSD



Class of Compounds
Hydrophilic, Ionizable
Analyzing Compounds Ethephon


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:

Application Detection:
ELSD Detection
UV Detection
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