Ethephon is an important compound for the agricultural industry. It can be corrosive, but metabolizes to ethylene quickly in plants. Ethephon was retained on SHARC 1 HILIC/anion-exclusion column. Retention was controlled by adjusting methanol in mobile phase to suppress hydrogen bonding.
| Column | Sharc 1, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN/MeOH – 90/10% to 50/50, 6 min |
| Buffer | Ammonia – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Hydrophilic, Ionizable |
| Analyzing Compounds | Ethephon |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsApplication Analytes:
EthephonApplication Detection:
ELSD DetectionUV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
