HPLC Separation of the Nonionic Surfactant Triton-X-100

Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. SHARC 1 HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved in anhydrous conditions by hydrogen bonding using acetonitrile (ACN) and methanol (MeOH) as the mobile phase with formic acid and ammonium formate (AmFm) as buffer. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (UV, ELSD, LC/MS, RI, CAD, etc.)


Column Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH - 100/0- 50/50%, 12  min
Buffer Formic Acid 0.1% , AmFm - 0.01%
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Nonionic Surfactant, Hydrophobic, Ionizable
Analyzing Compounds Triton-X-100
Application Analytes:
Triton X100