HPLC Separation of the Nonionic Surfactant Triton-X-100

Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. SHARC 1 HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved in anhydrous conditions by hydrogen bonding using acetonitrile (ACN) and methanol (MeOH) as the mobile phase with formic acid and ammonium formate (AmFm) as buffer. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (UV, ELSD, LC/MS, RI, CAD, etc.)


Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH – 100/0- 50/50%, 12  min
Buffer Formic Acid 0.1% , AmFm – 0.01%
Flow Rate 1.0 ml/min
Detection UV, 270 nm



Class of Compounds
Nonionic Surfactant, Hydrophobic, Ionizable
Analyzing Compounds Triton-X-100


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

Select options
Application Analytes:
Triton X100

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.