HPLC Separation of Pyrazine and Aminopyrazine on Sharc 1 Column

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HPLC Separation of Pyrazine and Aminopyrazine on Sharc 1 Column_1198


Column Sharc 1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 98/2%
Buffer Formic Acid – 0.5 % AmFm -0.05%
Flow Rate 1.0 ml/min
Detection UV 270 nm


Class of Compounds Hydrophilic,  Heterocyclic, Aromatic
Analyzing Compounds Pyrazine,  Aminopyrazine


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:
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