HPLC Separation of Phenoxymethylpenicillin

   

Phenoxymethylpenicillin (also known as penicillin V) is an antibiotic useful in the treatment of multiple bacterial infections such as those caused by Streptococcus pyogenes, Anthrax, Lyme Disease, Rheumatic fever, and blood infection prophylaxis in children with sickle cell diseases.  Legacy L1 was used to retain Phenoxymethylpenicillin by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

 

Condition

Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer NaH2PO4 pH 6.0 - 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm

Description

Class of Compounds  Drug,  Hydrophobic, Ionizable
Analyzing Compounds Phenoxymethylpenicillin
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SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Phenoxymethylpenicillin

Application Detection:
UV Detection