HPLC Separation of Phenoxymethylpenicillin


Phenoxymethylpenicillin (also known as penicillin V) is an antibiotic useful in the treatment of multiple bacterial infections such as those caused by Streptococcus pyogenes, Anthrax, Lyme Disease, Rheumatic fever, and blood infection prophylaxis in children with sickle cell diseases.  Legacy L1 was used to retain Phenoxymethylpenicillin by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.



Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer NaH2PO4 pH 6.0 - 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds  Drug,  Hydrophobic, Ionizable
Analyzing Compounds Phenoxymethylpenicillin
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Application Analytes:

Application Detection:
UV Detection