USP Methods for Chloramphenicol using a Legacy L1 Column

    Application Notes: Chloramphenicol is a common broad spectrum antibiotic developed in the 1940’s. According to the USP methods, chloramphenicol should contain no less than 97.0% and no more than 103% of chloramphenicol calculated on a dried basis. The USP HPLC method for the analysis of chloramphenicol was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Application Columns: Legacy L1 C18 HPLC column Application compounds: Chloramphenicol Mobile phase: MeOH/H2O/AcOH 45/55/0.1 Detection technique: UV Reference: USP 30: NF35


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O/AcOH 45/55/0.1
Buffer AcOH
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug,  Hydrophobic, Ionizable
Analyzing Compounds Chloramphenicol
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Application Analytes:

Application Detection:
UV Detection