USP Methods for Chloramphenicol using a Legacy L1 Column

    Application Notes: Chloramphenicol is a common broad spectrum antibiotic developed in the 1940’s. According to the USP methods, chloramphenicol should contain no less than 97.0% and no more than 103% of chloramphenicol calculated on a dried basis. The USP HPLC method for the analysis of chloramphenicol was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Application Columns: Legacy L1 C18 HPLC column Application compounds: Chloramphenicol Mobile phase: MeOH/H2O/AcOH 45/55/0.1 Detection technique: UV Reference: USP 30: NF35

Condition

Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O/AcOH 45/55/0.1
Buffer AcOH
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug,  Hydrophobic, Ionizable
Analyzing Compounds Chloramphenicol
 
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Application Analytes:
Chloramphenicol

Application Detection:
UV Detection