HPLC Analysis of Promethazine

  Promethazine is a drug used as an antihistamine, a sedative, and used to reduce motion sickness, and prescribed for insomnia. The reverse-phase column Legacy L1 was used to retain promethazine. comparisons between Legacy L1 and Phenomenex columns are available upon request.


Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN - 35%
Buffer NaH2PO4 pH 2.5 - 20 mM
Flow Rate 1.0 ml/min
Detection UV, 254 nm


Class of Compounds  Drug,  Hydrophobic, Ionizable
Analyzing Compounds Promethazine
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:

Application Detection:
UV Detection