HPLC Ananlysis of Hydrocortisone

 

Hydrocortisone is the drug form of the steroid hormone cortisol. Cortisol is an important biological molecule which affects blood sugar, metabolism and the immune system. It was retained on the reverse-phase column Legacy L1. Comparisons of Legacy L1 and Phenomenex columns are available by request.

Condition
Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN – 40%
Buffer NaH2PO4 pH 2.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV, 210 nm

Description
Class of Compounds
 Hormone,   Hydrophobic, Ionizable
Analyzing Compounds Hydrocortisone

&

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
Hydrocortisone
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.