HPLC Analysis of Cefepime


Cefepime is a fourth generation cephalosporin antibiotic usually reserved to treat severe nosocomial pneumonia and febrile neutropenia as well as multiple drug-resistant microorganisms such as Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. Legacy L1 was used to retain Cefepime by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.



Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeOH - 10%
Buffer NaH2PO4 pH 5.0- 5 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds  Drug,  Hydrophilic, Ionizable
Analyzing Compounds Cefepime
Application Analytes: