USP Methods for the Analysis of Hydrocortisone on a Legacy L1 Column

  Application Notes: Hydrocortisone is a naturally occurring hormone released during times of stress. Hydrocortisone has been synthesized and is used to treat diseases such as allergic reactions and skin conditions. According to the USP methods, hydrocortisone contains not less than 97 percent and not more than 102 percent of hydrocortisone calculated on the dried basis. The USP HPLC method for the separation of hydrocortisone was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Application Columns: Legacy L1 C18 HPLC column Application compounds: Hydrocortisone Mobile phase: MeCN/H2O 25:75 Detection technique: UV Reference: USP30: NF35


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase NaH2PO4 pH 7.0/MeCN 88/12
Buffer NaH2PO4
Flow Rate 1.0 ml/min
Detection UV, 254 nm


Class of Compounds Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Nitrofurantoin
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Application Analytes:

Application Detection:
UV Detection