HPLC Analysis of Paracetamol


Paracetamol (also known as acetaminophen) is a mild analgesic commonly used for relief from headaches and used in many cold and flu medicines. Legacy L1 was used to retain Paracetamol by reverse phase mechanism. Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.



Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeOH - 15%
Buffer AmAc pH 4.0- 42 mM
Flow Rate 1.0 ml/min
Detection UV, 257 nm


Class of Compounds  Drug,  Hydrophilic, Ionizable
Analyzing Compounds Paracetamol
Application Analytes:
Acetaminophen (Paracetamol)