Acetaminophen (Paracetamol)

CAS Number103-90-2
Molecular FormulaC8H9NO2
Molecular Weight151.165
InChI KeyRZVAJINKPMORJF-UHFFFAOYSA-N
LogP0.5
Synonyms
  • Acamol
  • Acephen
  • Acetaco
  • Acetamidophenol
  • Acetaminophen
  • Acetominophen
  • Algotropyl
  • Anacin 3
  • Anacin-3
  • Anacin3
  • APAP
  • Datril
  • Hydroxyacetanilide
  • N-(4-Hydroxyphenyl)acetanilide
  • N-Acetyl-p-aminophenol
  • p-Acetamidophenol
  • p-Hydroxyacetanilide
  • Panadol
  • Paracetamol
  • Tylenol

Applications:

HPLC Method for Separation of Acetaminophen and 4-Aminophenol on Primesep 100  Column

2023-01-31

HPLC Method for Separation of Acetaminophen and 4-Aminophenol on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Acetaminophen and 4-Aminophenol on Primesep 100 by SIELC Technologies
HPLC Method for Separation of Acetaminophen and 4-Aminophenol on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Separation of Acetaminophen and 4-Aminophenol

4-Aminophenol (also known as para-aminophenol or Rodinal) is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol or Tylenol). 4-Aminophenol can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 220 nm.

Condition

Column(Column variation not found)
Mobile PhaseMeCN – 10%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 220 nm
Peak Retention Time4.25, 5.51 min

Description

Class of CompoundsAlcohols, Phenols, Amines, Amine Salts, Dgru
Analyzing Compounds4-Aminophenol, Acetaminophen
Application Analytes:
4-Aminophenol
Acetaminophen (Paracetamol)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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HPLC Method for Separation of NyQuil Active Ingredients on BIST B+ Column

2021-10-18

Separation type: Bridge Ion Separation Technology, or BIST™


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen, Dextromethorphan, Pseudoephedrine, Doxylamine

Nyquil is a combination of medicines used to treat headaches, fever, body aches, cough, runny nose, sneezing, and sore throat caused by allergies, the common cold, or the flu. Nyquil contains the active ingredients acetaminophen, dextromethorphan HBr, doxylamine succinate, and sometimes, phenylephrine HCl. Acetaminophen is a pain reliever and fever reducer. Dextromethorphan is a cough suppressant. It affects the cough reflex in the brain that triggers coughing. Doxylamine is an antihistamine that reduces the effects of the natural chemical histamine in the body. Histamine can produce symptoms of sneezing, itching, watery eyes, and runny nose. Pseudoephedrine is used to relieve nasal congestion caused by colds, allergies, and hay fever. It is also used to temporarily relieve sinus congestion and pressure. All active compounds of Nyquil can be separated in HPLC on a BIST B+ column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 210 nm.

 

 

Condition

Column BIST B+, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 90-60%, 10 min
Buffer H2SO4 – 0.4%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Basic, Hydrophobic, Ionizable
Analyzing Compounds Acetaminophen, Dextromethorphan, Pseudoephedrine, Doxylamine

 

Application Analytes:

Acetaminophen (Paracetamol)
Dextromethorphan
Doxylamine
Pseudoephedrine (PSE)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

BIST B+

SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.

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HPLC Method for the Determination of Acetaminophen, Phenylephrine, and Ascorbic Acid on Primesep S2 Column

2021-10-05

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen, Phenylephrine, and Ascorbic Acid

Acetaminophen (or paracetamol) is one of the most popular over-the-counter painkillers all over the world (in the US it is best known under the brand name Tylenol). Ascorbic acid (also known as Vitamin C) helps your body build up muscle, cartilage, and other important building blocks in your body. Phenylephrine is a common over-the-counter decongestant, but can also be used for pupil dilation and hemorrhoid treatment. While all of these compounds are available at your local pharmacy, Vitamin C intake can reduce the body’s ability to break down acetaminophen.

All three of these compounds can be measured at low UV. Using a Primsep S normal phase column and a mobile phase consisting of water and acetonitrile (MeCN) with an Ammonium acetate (AmAc) buffer, these three compounds can be separated and UV detected at 270 nm. Varying the buffer concentration changes the order in which phenylephrine and ascorbic acid are retained. This method is compatible with Mass Spectrometry.

 

Condition

Column Primesep S2, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 97 to 70%
Buffer Gradient Ammonium Formate pH 3.0 – 10 to 50 mM
Flow Rate 1.0 ml/min
Detection UV 270 nm  MS- compatible mobile phase

Description

Class of Compounds Drug, Acid
Analyzing Compounds Acetaminophen, Phenylephrine, and Ascorbic Acid

 

Application Analytes:
Acetaminophen (Paracetamol)
Ascorbic Acid
Phenylephrine
Phenylephrine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep S2

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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HPLC Method for the Determination of Acetaminophen, Phenylephrine, and Ascorbic Acid on Primesep S Column

2021-10-05

Separation type: Liquid Chromatography Mixed-mode

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen, Phenylephrine, and Ascorbic Acid

Condition

Column Primesep S, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 80/20%
Buffer Ammonium acetate pH 5.0
Flow Rate 1.0 ml/min
Detection UV 270nm  MS- compatible mobile phase

Description

Class of Compounds Drug
Analyzing Compounds Acetaminophen, Phenylephrine, and Ascorbic Acid
Application Analytes:
Acetaminophen (Paracetamol)
Ascorbic Acid
Phenylephrine
Phenylephrine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep S

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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HPLC Separation of Acetaminophen, Caffeine and Pyrilamine maleate

2018-03-02

Acetaminophen is a p-aminophenol derivative with analgesic and antipyretic activities. It has weak anti-inflammatory properties, may cause liver, blood cell, and kidney damage. It is a nonprescription medication for mild-to-moderate pain and fever. Caffeine is a Central Nervous System (CNS) stimulant. It is an unregulated and legal drug in most parts of the world. It can be found in the seeds and leaves in a number of plants native in Africa, East Asia and South America. Pyrilamine maleate is a histamine H1 antagonist with hypnotic properties. It has multiple uses such as an anesthetic and is used against allergies. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain Acetaminophen, Caffeine and Pyrilamine maleate. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Analytes:
Acetaminophen (Paracetamol)
Caffeine
Maleate
Pyrilamine
Pyrilamine maleate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Newcrom R1

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

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HPLC Method for Analysis of Vicodin (Acetaminophen & Hydrocodone)

2016-05-10

78

Condition

Column Primesep 200, 3.2×100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 5-50%
Buffer Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Hydrocodone
Application Analytes:
Acetaminophen (Paracetamol)
Hydrocodone
Vicodin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep 200

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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HPLC Method for Analysis of Acetaminophen and Oxycodone

2016-05-06

11

Acetaminophen is a p-aminophenol derivative with analgesic and antipyretic activities. It has weak anti-inflammatory properties, may cause liver, blood cell, and kidney damage. It is a nonprescription medication for mild-to-moderate pain and fever.

Oxycodone is semi-synthetic opioid type drug that is used to help relieve moderate to severe pain. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Acetaminophen and Oxycodone. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Condition

Column Primesep 200, 3.2×100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 5-50%
Buffer Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Hydrocodone
Application Analytes:
Acetaminophen (Paracetamol)
Oxycodone

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep 200

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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HPLC Analysis of Paracetamol

2015-08-06

 

Paracetamol (also known as acetaminophen) is a mild analgesic commonly used for relief from headaches and used in many cold and flu medicines. Legacy L1 was used to retain Paracetamol by reverse phase mechanism. Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

 

Condition

Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeOH – 15%
Buffer AmAc pH 4.0- 42 mM
Flow Rate 1.0 ml/min
Detection UV, 257 nm

Description

Class of Compounds
 Drug,  Hydrophilic, Ionizable
Analyzing Compounds Paracetamol

 

Application Analytes:
Acetaminophen (Paracetamol)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
SIELC Legacy L1 HPLC column

Legacy L1

SIELC’s family of Legacy columns is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties so that you can easily replace older columns that are no longer available without having to significantly modify your method or SOPs.

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HPLC Separation of Caffeine, Benzoic acid and Acetaminophen

2015-08-06

Condition

Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeOH – 28%
Buffer Acetic Acid – 3%
Flow Rate 1.5 ml/min
Detection UV, 275 nm

Description

Class of Compounds
 Drug, Acid,  Hydrophilic, Hydrophobic, Ionizable
Analyzing Compounds Caffeine, Benzoic acid, Acetaminophen

&

Application Analytes:
Acetaminophen (Paracetamol)
Benzoic Acid
Caffeine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
SIELC Legacy L1 HPLC column

Legacy L1

SIELC’s family of Legacy columns is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties so that you can easily replace older columns that are no longer available without having to significantly modify your method or SOPs.

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Separation of Acetaminophen and 4-Aminophenol on Primesep C

2015-05-27

 

Acetaminophen and 4-Aminophenol ar both aminophenols. Acetominophen or paracetamol is a widely used analgesic and antipyretic. 4-Aminophenol is an intermediate in the synthesis of paracetamol and used in photography as a developer. Both compounds were retained and resolved on Primesep C. Primesep C is a mixed-mode HPLC column which uses reversed-phase and unique complex-forming groups to resolve compounds that might otherwise coelute.

 

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm

Description

Class of Compounds
 Drug,  Hydrophilic, Ionizable
Analyzing Compounds Acetaminophen,  4-Aminophenol

 

Application Analytes:
4-Aminophenol
Acetaminophen (Paracetamol)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep C

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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USP Methods for the Analysis of an Analgesic Mixture Using the Legacy L1 Column

2012-06-21

 

Application Notes: Acetametaphin, aspirin, and caffeine tablets contain not less than 90 percent and not more than 110 percent of the labeled amounts if acetametaphin, asprin, and caffeine according the USP methods. USP HPLC method for separation of acetaminophen, aspirin and caffeine was developed on Legacy L1 column according to US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column contains C18 ligands. Support for the material is a spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.

Application Columns: Legacy L1 C18 HPLC column

Application compounds: Acetaminophen, Aspirin, Caffeine, benzoic acid, and salicylic acid

Mobile phase: MeOH/H2O/AcOH 28/69/3

Detection technique: UV

Reference: USP30: NF35

Condition

Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O/AcOH 28/69/3
Buffer AcOH
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophobic, Ionizable
Analyzing Compounds Acetaminophen, Caffeine, Aspirin, Benzoic acid, Salicylic acid

 

Application Analytes:
Acetaminophen (Paracetamol)
Aspirin
Benzoic Acid
Caffeine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
SIELC Legacy L1 HPLC column

Legacy L1

SIELC’s family of Legacy columns is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties so that you can easily replace older columns that are no longer available without having to significantly modify your method or SOPs.

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HPLC Separation of NyQuil Active Ingredients

2011-06-04


NyQuil is a cough medication produced by Procter&Gamble. It consists of acetaminophen, pseudoephedrine, doxylamine and dextromethorphan. Most of the analytical HPLC methods include either use of ion-pairing reagent or a gradient elution. Mixed-mode chromatography allows to replace long gradient method with short isocratic method. All four compounds are well separated within 3-5 minutes. Methods can be optimized for analysis of other cough composition containing general cough suppressants and pain killers. Method is robust and being validated at several drug companies.

Condition

Column Primesep C, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Doxylamine, Dextromethorphan
Application Analytes:
Acetaminophen (Paracetamol)
Dextromethorphan
Doxylamine
Pseudoephedrine (PSE)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep C

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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HPLC Separation of Polar Drugs with MS-compatible Method

2010-10-12


Acetaminophen is an over-the-counter pain and fever reducer, and a major component of cold and flue remedies. Phenylephrine is a decongestant. Guaifenesin is a mucolytic agent used to relieve respiratory difficulties. These three compounds of cough medication were separated by mixed-mode chromatography on a Primesep C HPLC column. Retention and order of elution for phenylephrine can be changed by buffer concentration and buffer pH. Method can be used for analysis of cough and cold composition during the production and in QC/QA environment. Method is compatible with LC/MS and can be used to analyze these components in biological fluids. This generic HPLC method is robust and reproducible.

Condition

Column Primesep C, 3×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Guaifenesin
Application Analytes:
Acetaminophen (Paracetamol)
Guaifenesin
Phenylephrine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep C

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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Separation of Drugs in Mixed-Mode HPLC

2010-08-22


Obelisc R column can be successfully used for analysis of hydrophobic and hydrophilic basic and acidic drugs and corresponding counter-ions. This approach can be used for analysis of various cold and cough composition, pain killers, and other prescription and over-the-counter medications. Within pH 3-5, both cation- and anion mechanism are present on the column, along with hydrophobic interaction. Selectivity of this mixed-mode separation is controlled by the amount of acetonitrile, buffer concentration and buffer pH. Method is robust and reproducible, and can be used as a common HPLC method for analysis of drug molecules and compositions.

Condition

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Maleate, Chlorpheniramine, Dextromethorphan
Application Analytes:
Acetaminophen (Paracetamol)
Dextromethorphan

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Obelisc R

SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Effect of pH on Retention of Basic Compounds on Primesep C Columns

2010-01-13


Method for separation of components of cough medication shows how retention for acetaminophen, phenylephrine and guaifenesin is controlled. Order of elution for compounds can be changed based on composition of the mobile phase. The method is compatible with UV, ELSD and LC/MS and can be used for quantitation of drugs in formulation. Primesep C mixed-mode HPLC column is ideal candidate for analysis of cough compositions. Basic compounds are well retained in mixed-mode chromatography without use of ion-pairing reagents. Several companies validated this approach. The method is reproducible and robust and can be used in both production environments and R&D. This HPLC method can be adopted as general approach for analysis of basic compounds in various mixtures.

Condition

Column Primesep C, 3×50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Guaifenesin
Application Analytes:
Acetaminophen (Paracetamol)
Guaifenesin
Phenylephrine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep C

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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HPLC Separation of Drugs in Tylenol Cold and Cough Remedies

2008-08-22

Components of Tylenol Cold and Cough Remedy are separated on Obelisc R mixed-mode column. Method can be used to determine compounds in various cough and cold compositions. Neutral (acetaminophen), basic (chlorpheniramine, dextromethorphan and pseudoephedrine) and acidic (maleic acid/Maleate) components are analyzed with perfect peak shape and retention control. Method can be used in production, QC/QA and biological studies for quantitation of various components in pharmaceutical formulations (Advil, Tylenol, Dimetapp, Robitussin, NyQuil, etc)

Condition

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable, Neutral, Basic
Analyzing Compounds Maleate, Chlorpheniramine, Dextromethorphan, Pseudoephedrine,  Acetaminophen
Application Analytes:
Acetaminophen (Paracetamol)
Chlorpheniramine
Dextromethorphan
Maleate
Pseudoephedrine (PSE)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Obelisc R

SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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HPLC Separation of Acetaminophen, Phenylephrine, and Diphenhydramine

2006-03-03

Primesep C separates acetaminophen (paracetamol), phenylephrine, and diphenhydramine– common over-the-counter cold and flu medication ingredients such as Tylenol products—by either an acetonitrile or phosphoric acid gradient. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid with ultraviolet (UV) detection at 210 nm.

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Diphenhydramine
Application Analytes:
Acetaminophen (Paracetamol)
Diphenhydramine
Phenylephrine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep C

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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Separation of NyQuil Active Ingredients on Primesep C

2004-03-02

Primesep C separates the active ingredients in the over-the-counter cold medicine, NyQuil. Succinate and bromate co-elute early, but acetaminophen, pseudoephedrine, doxylamine, and dextromethorphan are baseline resolved. A combination of cation exchange, complex formation, and hydrophobic interactions separate this mixture with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 205 nm.

Condition

Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 205 nm

 

 

Description

Class of Compounds
Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Doxylamine, Dextromethorphan
Application Analytes:
Acetaminophen (Paracetamol)
Dextromethorphan
Doxylamine
Pseudoephedrine (PSE)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep C

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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