CAS Number | 93-14-1 |
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Molecular Formula | C10H14O4 |
Molecular Weight | 198.219 |
InChI Key | HSRJKNPTNIJEKV-UHFFFAOYSA-N |
LogP | 1.39 |
Synonyms |
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Separation type: Liquid Chromatography Mixed-mode
Terbutaline Sulfate is a β2 analyst typically used as a prescription-strength cough suppressant. Guaifenesin, also known as Glyceryl Guaiacolate, is a popular expectorant used to treat chest congestion. Ambroxol is a well-known expectorant that targets and breaks down phlegm. Together, these three medicinal compounds are typically found as the active ingredients in many cough syrups. They can be retained, analyzed, and separated on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Phosphoric acid (H3PO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm.
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Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 30/70% |
Buffer | H3PO4 – 0.05% |
Flow Rate | 1.0 ml/min |
Detection | UV 210 nm |
Peak Retention Time | 2.09, 3.28, 5.81 min |
Class of Compounds | Drugs |
Analyzing Compounds | Terbutaline Sulfate, Guaifenesin and Ambroxol |
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Notes: Guaifenesin is common, over the counter expectorant. Guaifenesin contain no less than 98 percent and not more than 102 percent of the labeled amount of guaifenesin calculated on a dried basis, according to the USP methods. the The USP HPLC method for the analysis of guaifenesin was developed on our Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A.
Application Columns: Legacy L1 C18 HPLC column
Application compounds: Guaifenesin, benzoic acid
Mobile phase: MeOH/H2O/AcOH 40:60:1.5
Detection technique: UV
Reference: USP 35- NF30
Column | Legacy L1, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeOH/H2O/AcOH 40/60/1.5 |
Buffer | NaH2PO4 |
Flow Rate | 1.0 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Drug, Antibiotics, Hydrophobic, Ionizable, Acid |
Analyzing Compounds | Guaifenesin, Benzoic acid |
SIELC’s family of Legacy columns is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties so that you can easily replace older columns that are no longer available without having to significantly modify your method or SOPs.
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Acetaminophen is an over-the-counter pain and fever reducer, and a major component of cold and flue remedies. Phenylephrine is a decongestant. Guaifenesin is a mucolytic agent used to relieve respiratory difficulties. These three compounds of cough medication were separated by mixed-mode chromatography on a Primesep C HPLC column. Retention and order of elution for phenylephrine can be changed by buffer concentration and buffer pH. Method can be used for analysis of cough and cold composition during the production and in QC/QA environment. Method is compatible with LC/MS and can be used to analyze these components in biological fluids. This generic HPLC method is robust and reproducible.
Column | Primesep C, 3×150 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | AmAc |
Flow Rate | 0.5 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
Analyzing Compounds | Acetaminophen, Pseudoephedrine, Guaifenesin |
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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Method for separation of components of cough medication shows how retention for acetaminophen, phenylephrine and guaifenesin is controlled. Order of elution for compounds can be changed based on composition of the mobile phase. The method is compatible with UV, ELSD and LC/MS and can be used for quantitation of drugs in formulation. Primesep C mixed-mode HPLC column is ideal candidate for analysis of cough compositions. Basic compounds are well retained in mixed-mode chromatography without use of ion-pairing reagents. Several companies validated this approach. The method is reproducible and robust and can be used in both production environments and R&D. This HPLC method can be adopted as general approach for analysis of basic compounds in various mixtures.
Column | Primesep C, 3×50 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | AmAc |
Flow Rate | 0.5 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
Analyzing Compounds | Acetaminophen, Pseudoephedrine, Guaifenesin |
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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