Ambroxol Hydrochloride

Ambroxolhydrochloride structural formula

CAS Number23828-92-4
Molecular FormulaC13H19Br2ClN2O
Molecular Weight414.571
InChI KeyQNVKOSLOVOTXKF-PFWPSKEQSA-N
LogP2.54
Synonyms
  • Ambroxolhydrochloride
  • (1r,4r)-4-{[(2-Amino-3,5-dibromophenyl)methyl]amino}cyclohexan-1-ol--hydrogen chloride (1/1)
  • Cyclohexanol, 4-[[(2-amino-3,5-dibromophenyl)methyl]amino]-, trans-, hydrochloride (1:1)
  • 23828-92-4
  • Ambroxol hydrochloride
  • trans-4-((2-Amino-3,5-dibromobenzyl)amino)cyclohexanol hydrochloride
  • EINECS 245-899-2
  • Mucolear
  • Mucosolvan
  • NA 872 hydrochloride
  • Mucoangin
  • Ambroxol lozenge
  • UNII-CC995ZMV90

Applications:

HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column

January 12, 2023

High Performance Liquid Chromatography (HPLC) Method for Separation of Bromhexine and Ambroxol on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column by SIELC Technologies
HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column by SIELC Technologies

Bromhexine is a popular mucolytic used to treat excessive mucus found in the respiratory system. Ambroxol is a well-known expectorant that targets and breaks down phlegm. These two mucus thinners can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm, 245 nm, and 315 nm.

High Performance Liquid Chromatography (HPLC) Method for Separation of Bromhexine and Ambroxol.

Condition

ColumnPrimesep 200, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 60/40%
BufferH3PO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210, 245, 315 nm
Peak Retention Time2.81, 4.22 min

Description

Class of CompoundsDrugs
Analyzing CompoundsBromhexine, Ambroxol

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ambroxol Hydrochloride
Bromhexine
Bromhexine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 Column

January 12, 2023

High Performance Liquid Chromatography (HPLC) Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 Column
HPLC Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 Column by SIELC Technologies

Terbutaline Sulfate is a β2 analyst typically used as a prescription-strength cough suppressant. Guaifenesin, also known as Glyceryl Guaiacolate, is a popular expectorant used to treat chest congestion. Ambroxol is a well-known expectorant that targets and breaks down phlegm. Together, these three medicinal compounds are typically found as the active ingredients in many cough syrups. They can be retained, analyzed, and separated on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Phosphoric acid (H3PO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm.

High Performance Liquid Chromatography (HPLC) Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol

.

Condition

ColumnPrimesep 200, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 30/70%
BufferH3PO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Peak Retention Time2.09, 3.28, 5.81 min

Description

Class of CompoundsDrugs
Analyzing CompoundsTerbutaline Sulfate, Guaifenesin and Ambroxol

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Ambroxol Hydrochloride
Guaifenesin
Terbutaline

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Ambroxolhydrochloride on Newcrom R1 HPLC column

February 16, 2018
Separation of Ambroxolhydrochloride on Newcrom C18 HPLC column

Ambroxolhydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Ambroxol Hydrochloride
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.