| CAS Number | 23828-92-4 |
|---|---|
| Molecular Formula | C13H19Br2ClN2O |
| Molecular Weight | 414.571 |
| InChI Key | QNVKOSLOVOTXKF-PFWPSKEQSA-N |
| LogP | 2.54 |
| Synonyms |
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Applications:
HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column
January 12, 2023
High Performance Liquid Chromatography (HPLC) Method for Separation of Bromhexine and Ambroxol on Primesep 200 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Bromhexine is a popular mucolytic used to treat excessive mucus found in the respiratory system. Ambroxol is a well-known expectorant that targets and breaks down phlegm. These two mucus thinners can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm, 245 nm, and 315 nm.
High Performance Liquid Chromatography (HPLC) Method for Separation of Bromhexine and Ambroxol.
Condition
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210, 245, 315 nm |
| Peak Retention Time | 2.81, 4.22 min |
Description
| Class of Compounds | Drugs |
| Analyzing Compounds | Bromhexine, Ambroxol |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Bromhexine
Bromhexine hydrochloride
HPLC Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 Column
January 12, 2023
High Performance Liquid Chromatography (HPLC) Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Terbutaline Sulfate is a β2 analyst typically used as a prescription-strength cough suppressant. Guaifenesin, also known as Glyceryl Guaiacolate, is a popular expectorant used to treat chest congestion. Ambroxol is a well-known expectorant that targets and breaks down phlegm. Together, these three medicinal compounds are typically found as the active ingredients in many cough syrups. They can be retained, analyzed, and separated on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Phosphoric acid (H3PO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm.
High Performance Liquid Chromatography (HPLC) Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol
.
Condition
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H3PO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 2.09, 3.28, 5.81 min |
Description
| Class of Compounds | Drugs |
| Analyzing Compounds | Terbutaline Sulfate, Guaifenesin and Ambroxol |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Guaifenesin
Terbutaline
Separation of Ambroxolhydrochloride on Newcrom R1 HPLC column
February 16, 2018
Ambroxolhydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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