Separation type: Liquid Chromatography Mixed-mode
Bromhexine is a popular mucolytic used to treat excessive mucus found in the respiratory system. Ambroxol is a well-known expectorant that targets and breaks down phlegm. These two mucus thinners can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm, 245 nm, and 315 nm.
Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 60/40% |
Buffer | H3PO4 – 0.05% |
Flow Rate | 1.0 ml/min |
Detection | UV 210, 245, 315 nm |
Peak Retention Time | 2.81, 4.22 min |
Class of Compounds | Drugs |
Analyzing Compounds | Bromhexine, Ambroxol |
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
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