High Performance Liquid Chromatography (HPLC) Method for Separation of Bromhexine and Ambroxol on Primesep 200 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Bromhexine is a popular mucolytic used to treat excessive mucus found in the respiratory system. Ambroxol is a well-known expectorant that targets and breaks down phlegm. These two mucus thinners can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm, 245 nm, and 315 nm.
High Performance Liquid Chromatography (HPLC) Method for Separation of Bromhexine and Ambroxol.
Condition
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210, 245, 315 nm |
| Peak Retention Time | 2.81, 4.22 min |
Description
| Class of Compounds | Drugs |
| Analyzing Compounds | Bromhexine, Ambroxol |
Application Analytes:
Ambroxol HydrochlorideBromhexine
Bromhexine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
