HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column

High Performance Liquid Chromatography (HPLC) Method for Separation of Bromhexine and Ambroxol on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column by SIELC Technologies
HPLC Method for Separation of Bromhexine and Ambroxol on Primesep 200 Column by SIELC Technologies

Bromhexine is a popular mucolytic used to treat excessive mucus found in the respiratory system. Ambroxol is a well-known expectorant that targets and breaks down phlegm. These two mucus thinners can be retained, analyzed, and separated on a Primesep 200 column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm, 245 nm, and 315 nm.

High Performance Liquid Chromatography (HPLC) Method for Separation of Bromhexine and Ambroxol.

Condition

ColumnPrimesep 200, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 60/40%
BufferH3PO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210, 245, 315 nm
Peak Retention Time2.81, 4.22 min

Description

Class of CompoundsDrugs
Analyzing CompoundsBromhexine, Ambroxol

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ambroxol Hydrochloride
Bromhexine
Bromhexine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.