HPLC Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 Column

High Performance Liquid Chromatography (HPLC) Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 Column
HPLC Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol on Primesep 200 Column by SIELC Technologies

Terbutaline Sulfate is a β2 analyst typically used as a prescription-strength cough suppressant. Guaifenesin, also known as Glyceryl Guaiacolate, is a popular expectorant used to treat chest congestion. Ambroxol is a well-known expectorant that targets and breaks down phlegm. Together, these three medicinal compounds are typically found as the active ingredients in many cough syrups. They can be retained, analyzed, and separated on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Phosphoric acid (H3PO4) ionic modifier. This analysis method can be detected in the low UV regime at 210 nm.

High Performance Liquid Chromatography (HPLC) Method for Separation of Terbutaline Sulfate, Guaifenesin and Ambroxol

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Condition

ColumnPrimesep 200, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 30/70%
BufferH3PO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Peak Retention Time2.09, 3.28, 5.81 min

Description

Class of CompoundsDrugs
Analyzing CompoundsTerbutaline Sulfate, Guaifenesin and Ambroxol

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ambroxol Hydrochloride
Guaifenesin
Terbutaline
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.