HPLC Method for Ambroxol Hydrochloride, Guaifenesin, Terbutaline, Ambroxol on Primesep 200 by SIELC Technologies

Terbutaline Sulfate is a salt form of Terbutaline, which is a β₂ adrenergic receptor agonist with the chemical formula C₁₂H₁₉NO₃. It is typically perscribed as an inhaler to assist with managing asthma symptoms as well as a tocolytic to relay preterm labor.. It is prohibited from use by the World Anti-Doping Agency without a Therapeutic Use Exemption (TUE) and is only allowed to be administered by inhalation. You can find detailed UV spectra of Terbutaline and information about its various lambda maxima by visiting the following link.

Guaifenesin, also known as glyceryl guaiacolate, is a drug with the chemical formula C₁₀H₁₄O₄. It is used to remove sputum, or phlegm, from the respiratory tract. It works through stimulating the gastric mucosa, which leads to a higher volume and reduced viscosity of secretions in the trachea and the bronchi. You can find detailed UV spectra of Guaifenesin and information about its various lambda maxima by visiting the following link.

Ambroxol is a well-known expectorant with the chemical formula C₁₃H₁₈Br₂N₂O. It targets and breaks down phlegm through inhibiting neuronal Na+ channels. It also has anti-inflammatory properties. It has not yet been approved for use by the USA Food and Drug Administration. You can find detailed UV spectra of Ambroxol and information about its various lambda maxima by visiting the following link.

Together, these three medicinal compounds are typically found as the active ingredients in many cough syrups.

Ambroxol Hydrochloride, Guaifenesin, Terbutaline, Ambroxol can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV. This analysis method can be detected in the low UV regime at 210 nm.

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