HPLC Method for Analysis of Codeine and Guaifenesin in Cough syrup on Primesep 100  Column

HPLC Method for Codeine, Guaifenesin on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Codeine, Guaifenesin

Codeine is a pain reliever and cough suppressant. It belongs to the opioid family (like morphine, but milder). Guaifenesin is an expectorant, which helps loosen mucus in your chest so you can cough it up more easily.

Codeine, Guaifenesin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 270 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 30%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 270 nm

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsDrug
Analyzing CompoundsCodeine, Guaifenesin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Codeine
Guaifenesin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.