CAS Number | 76-57-3 |
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Molecular Formula | C18H21NO3 |
Molecular Weight | 299.370 |
InChI Key | OROGSEYTTFOCAN-DNJOTXNNSA-N |
LogP | 0.761 |
Synonyms |
|
Column | Primesep C, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 35/65% |
Buffer | TEAPh Ph 3.5 – 15 Mm |
Flow Rate | 1.0 ml/min |
Detection | UV, 210 nm |
Class of Compounds |
Drug, Preservatives, Base, Hydrophobic, Ionizable |
Analyzing Compounds | Codeine, Methyl Paraben, Propyl Paraben |
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsColumn | Primesep C, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 35/65% |
Buffer | TEAPh Ph 3.5 – 15 Mm |
Flow Rate | 1.0 ml/min |
Detection | UV, 210 nm |
Class of Compounds |
Drug, Preservatives, Base, Hydrophobic, Ionizable |
Analyzing Compounds | Codeine, Methyl Paraben, Propyl Paraben |
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsCodeine and two parabens are separated by mixed-mode chromatography. Codeine is retained by reversed-phase and cation-exchange mechanisms and parabens are retained by reversed-phase mechanism. The retention time of codeine can be adjusted by changing the amount of acetonitrile, buffer concentration, and buffer pH. The mobile phase for this column and its separation are fully compatible with UV, ELSD, LC/MS and prep chromatography, various organic and inorganic acids, and corresponding buffers can be used.
Codeine is a hydrophobic basic drug which is used in many drug compositions as an analgetic, antitussive, anxiolytic, and sedative agent. Codeine is widely used as a moderate pain and cough reliever. It is usually part of complex composition and comes in the form of a tablet or syrup. Several preservatives are used in most of the drug composition and include parabens and benzoates. The mixture of codeine, methyl and propyl parabens was separated on Primesep C mixed-mode reversed-phase cation-exchange column. Codeine is retained by reversed-phase and cation-exchange mechanisms and parabens are retained by reversed-phase mechanism. No ion-pairing reagent is required since Primesep C mixed-mode stationary phase has an ion-pairing reagent attached to the surface.
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select options
Codeine, oxycodone, and hydrocodone are analgesics with hydrophobic and basic properties. All three compounds are used as strong pain killers and cough suppressant. These compounds are controlled substances according to DEA. We developed a simple method for separation of these narcotics on Primesep 100 and Primesep 200 mixed-mode columns. All three compounds are separated by reversed-phase and cation-exchange mechanism, based on the difference in hydrophobic and ionic properties. This HPLC approach can be used to develop and validate a generic method for analysis of analgesics and other drugs. Compounds are monitored by common detection techniques.
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsThe Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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