HPLC Separation of Drugs in Tylenol Cold and Cough Remedies

Components of Tylenol Cold and Cough Remedy are separated on Obelisc R mixed-mode column. Method can be used to determine compounds in various cough and cold compositions. Neutral (acetaminophen), basic (chlorpheniramine, dextromethorphan and pseudoephedrine) and acidic (maleic acid/Maleate) components are analyzed with perfect peak shape and retention control. Method can be used in production, QC/QA and biological studies for quantitation of various components in pharmaceutical formulations (Advil, Tylenol, Dimetapp, Robitussin, NyQuil, etc)


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable, Neutral, Basic
Analyzing Compounds Maleate, Chlorpheniramine, Dextromethorphan, Pseudoephedrine,  Acetaminophen

Application Analytes:
Acetaminophen (Paracetamol)
Pseudoephedrine (PSE)