HPLC Method for Separation of Acetaminophen and 4-Aminophenol on Primesep 100 by SIELC Technologies
4-Aminophenol, also known as para-aminophenol, and Rodinal is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol and Tylenol). It has the chemical formula H2NC6H4OH. It is usually found as a white, moderately soluble in alcohol, powder, but it can be recrystallized in hot water. You can find detailed UV spectra of 4-Aminophenol and information about its various lambda maxima by visiting the following link.
Acetaminophen, also known as Paracetamol, is a medication with the molecular formula C8H9NO2. It is an over-the-counter pain killer that also reduces fever. Unlike Ibuprofen and naproxen, it is not an anti-inflammatory drug. You can find detailed UV spectra of Acetaminophen and information about its various lambda maxima by visiting the following link.
4-Aminophenol, Acetaminophen (Paracetamol) can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an sulfuric acid (H2SO4) or perchloric acid (HClO4) buffer. This analysis method can be detected in the UV regime at 220 nm.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 – 0.2% or HClO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
| Peak Retention Time | 4.25, 5.51 min |
Description
| Class of Compounds | Alcohols, Phenols, Amines, Amine Salts, Dgru |
| Analyzing Compounds | 4-Aminophenol, Acetaminophen (Paracetamol) |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Acetaminophen (Paracetamol)
