HPLC Method for the Determination of Acetaminophen, Phenylephrine, and Ascorbic Acid on Primesep S Column

HPLC Method for Acetaminophen (Paracetamol), Phenylephrine, Ascorbic Acid, Phenylephrine hydrochloride on Primesep S by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen, Phenylephrine, and Ascorbic Acid

Acetaminophen, also known as Paracetamol, is a medication with the molecular formula C8H9NO2. It is an over-the-counter pain killer that also reduces fever. Unlike Ibuprofen and naproxen, it is not an anti-inflammatory drug.

Phenylephrine is a common over-the-counter decongestant, but can also be used for pupil dilation and hemorrhoid treatment. It’s chemical formula is C9H13NO2.

Ascorbic Acid is a vitamin with the molecular formula C6H8O6. Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus.

You can find detailed UV spectra of Acetaminophen and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Ascorbic Acid and information about its various lambda maxima by visiting the following link.

Acetaminophen (Paracetamol), Phenylephrine, Ascorbic Acid, Phenylephrine hydrochloride can be retained and analyzed using the Primesep S stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium acetate buffer. Detection is performed using UV.

Condition

Column Primesep S, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 80/20%
Buffer Ammonium acetate pH 5.0
Flow Rate 1.0 ml/min
Detection UV 270nm  MS- compatible mobile phase

Description

Class of Compounds Drug
Analyzing Compounds Acetaminophen (Paracetamol), Phenylephrine, Ascorbic Acid, Phenylephrine hydrochloride

Application Column

Primesep S

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acetaminophen (Paracetamol)
Ascorbic Acid
Phenylephrine
Phenylephrine hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.