Excedrin is over-the-counter pain reliever containing acetaminophen, caffeine and aspirin as active ingredients of this drug composition. Acetaminophen (paracetamol) is used as analgesic and pain reliever. It is a neutral compound with low hydrophobicity. Aspirin or acetylsalicylic acid is used as analgesic and anti-inflammatory component of many OTC compositions. It is weakly acidic and slightly hydrophobic compound. Caffeine is xanthine alkaloid which is psychoactive stimulant drug. All four compounds are separated on mixed-mode Primesep 100 HPLC column with acetonitrile/water/TFA mobile phase. In this HPLC application compounds are retained by reversed phase mechanism. This HPLC method is short and robust.
| Column | Primesep 100, 3.2×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Benzoic acid, Acetaminophen, Caffeine, Aspirin |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAspirin
Benzoic Acid
Caffeine
UV Detection
