Separation of Acetaminophen and 4-Aminophenol on Primesep C

  Acetaminophen and 4-Aminophenol ar both aminophenols. Acetominophen or paracetamol is a widely used analgesic and antipyretic. 4-Aminophenol is an intermediate in the synthesis of paracetamol and used in photography as a developer. Both compounds were retained and resolved on Primesep C. Primesep C is a mixed-mode HPLC column which uses reversed-phase and unique complex-forming groups to resolve compounds that might otherwise coelute.  


Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds  Drug,  Hydrophilic, Ionizable
Analyzing Compounds Acetaminophen,  4-Aminophenol
Application Analytes:
Acetaminophen (Paracetamol)