HPLC Method for Analysis of Acetaminophen and Oxycodone

11 Acetaminophen is a p-aminophenol derivative with analgesic and antipyretic activities. It has weak anti-inflammatory properties, may cause liver, blood cell, and kidney damage. It is a nonprescription medication for mild-to-moderate pain and fever. Oxycodone is semi-synthetic opioid type drug that is used to help relieve moderate to severe pain. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Acetaminophen and Oxycodone. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Condition

Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%
Buffer Gradient H3PO4 - 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Hydrocodone
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Acetaminophen (Paracetamol)
Oxycodone

Application Detection:
UV Detection