Primesep C separates the active ingredients in the over-the-counter cold medicine, NyQuil. Succinate and bromate co-elute early, but acetaminophen, pseudoephedrine, doxylamine, and dextromethorphan are baseline resolved. A combination of cation exchange, complex formation, and hydrophobic interactions separate this mixture with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 205 nm.
| Column | Primesep C, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | TEAPh |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 205 nm |
| Class of Compounds |
Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Acetaminophen, Pseudoephedrine, Doxylamine, Dextromethorphan |
Application Column
Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsDextromethorphan
Doxylamine
Pseudoephedrine (PSE)
