HPLC Separation of Caffeine, Benzoic acid and Acetaminophen


Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeOH – 28%
Buffer Acetic Acid – 3%
Flow Rate 1.5 ml/min
Detection UV, 275 nm


Class of Compounds
 Drug, Acid,  Hydrophilic, Hydrophobic, Ionizable
Analyzing Compounds Caffeine, Benzoic acid, Acetaminophen


SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
SIELC Legacy L1 HPLC column

Legacy L1

SIELC’s family of Legacy columns is based on United States Pharmacopeia’s (USP) published chromatographic methods and procedures. A plethora of brands have columns used in USP reference standards and methods, and USP has created different designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties so that you can easily replace older columns that are no longer available without having to significantly modify your method or SOPs.

Select options
Application Analytes:
Acetaminophen (Paracetamol)
Benzoic Acid

Application Detection:
UV Detection