HPLC Separation of Acetaminophen, Caffeine and Pyrilamine maleate

HPLC Method for Acetaminophen (Paracetamol), Caffeine, Pyrilamine maleate, Maleate, Pyrilamine on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen (Paracetamol), Caffeine, Pyrilamine maleate, Maleate, Pyrilamine 

Acetaminophen is a p-aminophenol derivative with analgesic and antipyretic activities. It has weak anti-inflammatory properties, may cause liver, blood cell, and kidney damage. It is a nonprescription medication for mild-to-moderate pain and fever. Caffeine is a Central Nervous System (CNS) stimulant. It is an unregulated and legal drug in most parts of the world. It can be found in the seeds and leaves in a number of plants native in Africa, East Asia and South America. Pyrilamine maleate is a histamine H1 antagonist with hypnotic properties. It has multiple uses such as an anesthetic and is used against allergies. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain Acetaminophen, Caffeine and Pyrilamine maleate. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

ColumnNewcrom R1, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 10%
BufferTriethanolamine
Flow Rate0.5 ml/min
DetectionUV 250nm
Class of CompoundsAnalgesic, Antipyretic
Analyzing CompoundsAcetaminophen (Paracetamol), Caffeine, Pyrilamine maleate, Maleate, Pyrilamine

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acetaminophen (Paracetamol)
Caffeine
Maleate
Pyrilamine
Pyrilamine maleate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.