Application Notes: Acetametaphin, aspirin, and caffeine tablets contain not less than 90 percent and not more than 110 percent of the labeled amounts if acetametaphin, asprin, and caffeine according the USP methods. USP HPLC method for separation of acetaminophen, aspirin and caffeine was developed on Legacy L1 column according to US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column contains C18 ligands. Support for the material is a spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.
Application Columns: Legacy L1 C18 HPLC column
Application compounds: Acetaminophen, Aspirin, Caffeine, benzoic acid, and salicylic acid
Mobile phase: MeOH/H2O/AcOH 28/69/3
Detection technique: UV
Reference: USP30: NF35
| Column | Legacy L1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O/AcOH 28/69/3 |
| Buffer | AcOH |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Hydrophobic, Ionizable |
| Analyzing Compounds | Acetaminophen, Caffeine, Aspirin, Benzoic acid, Salicylic acid |
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
Select optionsAspirin
Benzoic Acid
Caffeine
