| CAS Number | 5957-75-5 |
|---|---|
| Molecular Formula | C21H30O2 |
| Molecular Weight | 311.5 |
| InChI Key | HCAWPGARWVBULJ-UHFFFAOYSA-N |
| LogP | 5.7 |
| Synonyms |
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Applications:
HPLC Determination of CBD, CBN, THC, 8-THC and THCA on Cannsep C Column
June 14, 2021
Separation type: Liquid Chromatography Reverse Phase.
HPLC Method for Analysis of Cannabidiol (CBD), Cannabinol (CBN), Tetrahydrocannabinol (THC), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC), Tetrahydrocannabinolic acid (THCA-A) on Cannsep C by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, THC, 8-THC and THCA.
Δ8-THC, Δ9-THC, CBD, THCA and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH), Acetonitrile (MeCN, ACN), and water with sulfuric acid (H2SO4) as buffer and UV detected at 210nm.
| Column | Cannsep C, 2.1 x 100 mm, 3 µm, 100 A |
| Mobile Phase | MeCN/MeOH/H2O – 45/40/15% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol (THC), CBD, CBN, Δ9-THC, Δ8-THC, Tetrahydrocannabinolic acid (THCA) |
Application Column
Cannsep C
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
HPLC Separation of CBD, CBN, delta-9 THC, delta-8 THC on Cannsep C Column
April 30, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, delta-9 THC, delta-8 THC
Cannabidiol (CBD), cannabinol (CBN), and delta-9-Tetrahydrocannabinol (delta-9 THC) are three of the most active cannabinoids in cannabis. Delta-8-tetrahydrobannabinol also occurs naturally, but in such small amounts that it is often cheaper and easier to synthesize it in the laboratory from CBD. Both forms of THC are the strongest of the cannabinoids and are the main cause of the psychoactive effects of cannabis. CBD, while the 2nd most active component, does not cause the same effects by itself, but studies show it may have other neurologic and psychiatric benefits. There has been a lot less research conducted on CBN and its potential health benefits, but it is a product of the breakdown of THC and works on the same receptors (albeit with different effects) as CBD.
These cannabinoids can be detected in the low UV regime. Using a Cannsep C reverse-phase column and a mobile phase consisting of water and methanol (MeOH) with no buffer, CBD, CBN, delta-9 THC, and delta-8 THC can be separated, measured, and analyzed. This analysis method can be UV detected at 275 nm with high resolution.
| Column | Cannsep C, 2.1×100 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 82/18% |
| Buffer | No |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 275 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol (THC), Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (THC), |
Application Column
Cannsep C
The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.
Select optionsCannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
HPLC Determination of Δ8-THC, CBD, Δ9-THC, CBN, on Primesep N Column
May 15, 2020
Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a normal-phase Primesep N column using a mobile phase consisting of hexane and ethyl acetate without the need for a buffer with UV detection at 275nm.
| Column | Primesep N , 3.2×150 mm, 3 µm, 100A |
| Mobile Phase | Hexane/Ethyl Acetate – 97/3% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 275 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Delta -8-Tetrahydrocannabinol, Δ8-THC, Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol, Δ9-THC, Cannabinol (CBN) |
Application Column
Primesep N
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
HPLC Determination of CBD, CBN, Δ9-THC, Δ8-THC on Cannsep C Column
May 14, 2020
Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.
| Column | Cannsep C, 3.2×150 mm, 3 µm, 100A |
| Mobile Phase | MeOH -80%, |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol (THC), CBD, CBN, Δ9-THC, Δ8-THC |
Application Column
Cannsep C
The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.
Select optionsCannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
HPLC Separation of Ten Cannabinoids on Cannsep A Column
January 13, 2020
| Column | Cannsep A, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN 50-70% , 20 min |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A) |
Application Column
Cannsep A
The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.
Select optionsCannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)
