Tetrahydrocannabinol (THC)

CAS Number1972-08-3
Molecular FormulaC21H30O2
Molecular Weight314.469
InChI KeyCYQFCXCEBYINGO-IAGOWNOFSA-N
LogP7.22
Synonyms
  • Tetrahydrocannabinol
  • (6aR,10aR)-6,6,9-Trimethyl-3-pentyl-6a,7,8,10a-tetrahydro-6H-dibenzo[b,d]pyran-1-ol
  • 6H-Dibenzo[b,d]pyran-1-ol, 6a,7,8,10a-tetrahydro-6,6,9-trimethyl-3-pentyl-, (6aR,10aR)-
  • 1972-08-3
  • THC
  • 1-trans-delta-9-Tetrahydrocannabinol
  • (6aR,10aR)-6,6,9-Trimethyl-3-pentyl-6a,7,8,10a-tetrahydro-6H-dibenzo[b,d]pyran-1-ol
  • Cannabinoids, THC
  • QCD 84924
  • SP 104
  • 6H-Dibenzo[b,d]piran-1-ol, 6a,7,8,10a-tetrahidro-6,6,9-trimetil-3-pentil-, (6aR,10aR)-
  • (6AR,10aR)-6,6,9-trimethyl-3-pentyl-6a,7,8,1Oa-tetrahydro-6H-benzo[c]chromen-1-ol
  • tetrahydro-6,6,9-trimethyl-3-pentyl-6H-dibenzo[b,d]pyran-1-o
  • 6H-Dibenzo[b,d]pyran-1-ol, 6a,7,8,10a-tetrahydro-6,6,9-trimethyl-3-pentyl-, (6aR,10aR)-
  • (-)-3,4-trans-delta1-Tetrahydrocannabinol
  • (-)-trans-delta1-Tetrahydrocannabinol
  • (-)-trans-delta9-Tetrahydrocannabinol
  • (-)-trans-delta9-THC
  • (-)-delta1-Tetrahydrocannabinol
  • (-)-delta9-Tetrahydrocannabinol
  • (-)-delta9-trans-Tetrahydrocannabinol
  • (l)-delta1-Tetrahydrocannabinol
  • 6H-Dibenzo[b,d]pyran-1-ol, 6a,7,8,10a-tetrahydro-6,6,9-trimethyl-3-pentyl-, (6aR-trans)-
  • Abbott 40566
  • Cannabinol, tetrahydro-
  • Cannabinol, delta1-tetrahydro-
  • Dronabinol
  • l-trans-delta9-Tetrahydrocannabinol
  • l-delta1-trans-Tetrahydrocannabinol
  • Marinol
  • NSC 134454
  • trans-(-)-delta9-Tetrahydrocannabinol
  • trans-delta9-Tetrahydrocannabinol
  • delta1-Tetrahydrocannabinol
  • delta9-Tetrahydrocannabinol
  • delta9-trans-Tetrahydrocannabinol
  • Cannabinol, 1-trans-delta(sup 9)-tetrahydro-
  • (l)-delta(sup 1)-Tetrahydrocannabinol
  • delta(sup 1)-Tetrahydrocannabinol
  • (-)-delta(sup 1)-3,4-trans-Tetrahydrocannabinol
  • 1-trans-delta(sup 9)-Tetrahydrocannabinol
  • delta(sup 9)-Tetrahydrocannabinol
  • delta(sup 1)-Thc
  • delta(sup 9)-Thc
  • delta(9)-Tetrahydrocannibinol
  • Tetrahydro-6,6,9-trimethyl-3-pentyl-6H-dibenzo(b,d)pyran-1-ol
  • 6,6,9-Trimethyl-3-pentyl-7,8,9,10-tetrahydro-6H-dibenzo(b,d)pyran-1-ol
  • Deltanyne
  • Tetrahydrocannabinols (-)-delta1-3,4-trans-form
  • UNII-7J8897W37S
  • delta-9 Tetrahydrocannabinol
  • Namisol
  • 3-Pentyl-6,6,9-trimethyl-6a,7,8,10a-tetrahydro-6H-dibenzo(b,d)pyran-1-ol
  • 6,6,9-Trimethyl-3-pentyl-6a,7,8,10a-tetrahydro-6H-benzo[c]chromen-1-ol
  • 6H-Dibenzo(b,d)pyran-1-ol, 6a,7,8,10a-tetrahydro-6,6,9-trimethyl-3-pentyl-, (6aR,10aR)-
  • Delta(1)-tetrahydrocannabinol
  • Delta(9)-THC
  • Delta(9)-tetrahydrocannabinol
  • Dronabinolum
  • 1363-19-5
  • 5957-27-7
  • 14146-29-3
  • 14146-43-1
  • 26108-45-2

Applications:

HPLC Determination of CBD, CBN, THC, 8-THC and THCA on Cannsep C Column

June 14, 2021

Separation type: Liquid Chromatography Reverse Phase.

HPLC Method for Analysis of Cannabidiol (CBD), Cannabinol (CBN), Tetrahydrocannabinol (THC), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC), Tetrahydrocannabinolic acid (THCA-A) on Cannsep C by SIELC Technologies

HPLC Determination of CBD, CBN, THC, 8-THC and THCA on Cannsep C Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, THC, 8-THC and THCA.

Δ8-THC, Δ9-THC, CBD, THCA and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH), Acetonitrile (MeCN, ACN), and water with sulfuric acid (H2SO4) as buffer and UV detected at 210nm.



ColumnCannsep C, 2.1 x 100 mm, 3 µm, 100 A
Mobile PhaseMeCN/MeOH/H2O – 45/40/15%
BufferH2SO4 – 0.2%
Flow Rate0.2 ml/min
DetectionUV, 200 nm
Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidiol (CBD),  Delta-9-Tetrahydrocannabinol (THC), CBD, CBN, Δ9-THC, Δ8-THC, Tetrahydrocannabinolic acid  (THCA)

Application Column

Cannsep C

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A

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Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of THC in THC Infused Gummy Bear on Cannsep C Column

January 24, 2020

Condition
Column Cannsep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 60-80%, 25 min, 5 min hold
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD),  Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabidiol (CBD)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Ten Cannabinoids on Cannsep C Column

January 13, 2020

Condition
Column Cannsep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 60-80% , 20 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD),  Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Ten Cannabinoids on Cannsep B Column

January 13, 2020

Condition
Column Cannsep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 50-70% , 25 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD),  Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep B

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Ten Cannabinoids on Cannsep A Column

January 13, 2020

Condition
Column Cannsep A, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 50-70% , 20 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD),  Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep A

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabidiol (CBD)
Cannabidiolic acid (CBDA)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Tetrahydrocannabivarin (THCV)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Fast Separation of Cannabinoids on Mixed-Mode HPLC Column Cannsep B

December 5, 2013

With legalization of marijuana in much of the United States, more and more attention is paid to developing quick, robust and reliable method for quantitation of cannabinoids in marijuana plants and related edible products. The main challenge lies in sample preparation and quantitative analysis of four major cannabinoids: cannabidiol, cannabinol, tetrahydrocannabinol and tetrahydrocannabinolic acid. All compounds are hydrophobic with only THCA (THC-A) and cannabidiolic acid (CBDA) being an acid. Hydrophobic interaction is the main interaction in the separation of cannabinoids on reverse phase columns. On our mixed mode columns acidic molecules retained by ion-exchange mechanism  in addition to hydrophobic. Method can be used for analysis and prep separation of cannabinoids in marijuana plants, seeds and other cannabis-based products. Extraction of cannabinoids is the main procedure in sample preparation. Extraction can be done with organic solvents like chloroform, methanol, ethanol, acetonitrile.  Cannabinoids can be monitored by UV and LC/MS. Mixed-mode columns containing polar embedded groups provide different selectivity than regular reversed-phase columns.

 

Condition
Column Cannsep B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 40-60% , 20 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidivarin (CBDV), Tetrahydrocannabivarin (THCV), Cannabidiol (CBD),  Delta-9-Tetrahydrocannabinol (THC), Cannabigerol (CBG), Cannabichromene (CBC), Tetrahydrocannabinolic acid (THCA-A)

 

Application Column

Cannsep B

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabichromene (CBC)
Cannabidiol (CBD)
Cannabidivarin (CBDV)
Cannabigerol (CBG)
Cannabinol (CBN)
Tetrahydrocannabinol (THC)
Tetrahydrocannabinolic acid (THCA-A)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.