Cannabidiol (CBD)

Separation type: Liquid Chromatography Reverse Phase.

HPLC Method for Analysis of Cannabidiol (CBD), Cannabinol (CBN), Tetrahydrocannabinol (THC), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC), Tetrahydrocannabinolic acid (THCA-A) on Cannsep C by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, THC, 8-THC and THCA.

Δ8-THC, Δ9-THC, CBD, THCA and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH), Acetonitrile (MeCN, ACN), and water with sulfuric acid (H2SO4) as buffer and UV detected at 210nm.

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, delta-9 THC, delta-8 THC

Cannabidiol (CBD), cannabinol (CBN), and delta-9-Tetrahydrocannabinol (THC) are three of the most active cannabinoids in cannabis. THC is the strongest of the three and is the main cause of the psychoactive effects of cannabis. CBD, while the 2nd most active component, does not cause the same effects by itself, but studies show it may have other neurologic and psychiatric benefits. There has been a lot less research conducted on CBN and its potential health benefits, but it is a product of the breakdown of THC and works on the same receptors (albeit with different effects) as CBD.
These cannabinoids can be detected in the low UV regime. Using a Cannsep C reverse-phase column and a mobile phase consisting of water, methanol (MeOH) and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, CBD, CBN, and THC can be separated, measured, and analyzed. This analysis method can be UV detected at 210 nm with high resolution, even in real-world samples.

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, delta-9 THC, delta-8 THC

Cannabidiol (CBD), cannabinol (CBN), and delta-9-Tetrahydrocannabinol (delta-9 THC) are three of the most active cannabinoids in cannabis. Delta-8-tetrahydrobannabinol also occurs naturally, but in such small amounts that it is often cheaper and easier to synthesize it in the laboratory from CBD. Both forms of THC are the strongest of the cannabinoids and are the main cause of the psychoactive effects of cannabis. CBD, while the 2nd most active component, does not cause the same effects by itself, but studies show it may have other neurologic and psychiatric benefits. There has been a lot less research conducted on CBN and its potential health benefits, but it is a product of the breakdown of THC and works on the same receptors (albeit with different effects) as CBD.
These cannabinoids can be detected in the low UV regime. Using a Cannsep C reverse-phase column and a mobile phase consisting of water and methanol (MeOH) with no buffer, CBD, CBN, delta-9 THC, and delta-8 THC can be separated, measured, and analyzed. This analysis method can be UV detected at 275 nm with high resolution.

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Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a normal-phase Primesep N column using a mobile phase consisting of hexane and ethyl acetate without the need for a buffer with UV detection at 275nm.

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Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

With legalization of marijuana in much of the United States, more and more attention is paid to developing quick, robust and reliable method for quantitation of cannabinoids in marijuana plants and related edible products. The main challenge lies in sample preparation and quantitative analysis of four major cannabinoids: cannabidiol, cannabinol, tetrahydrocannabinol and tetrahydrocannabinolic acid. All compounds are hydrophobic with only THCA (THC-A) and cannabidiolic acid (CBDA) being an acid. Hydrophobic interaction is the main interaction in the separation of cannabinoids on reverse phase columns. On our mixed mode columns acidic molecules retained by ion-exchange mechanism  in addition to hydrophobic. Method can be used for analysis and prep separation of cannabinoids in marijuana plants, seeds and other cannabis-based products. Extraction of cannabinoids is the main procedure in sample preparation. Extraction can be done with organic solvents like chloroform, methanol, ethanol, acetonitrile.  Cannabinoids can be monitored by UV and LC/MS. Mixed-mode columns containing polar embedded groups provide different selectivity than regular reversed-phase columns.