HPLC Determination of CBD, CBN, THC in 1:1 Citrus Lime Tonic on Canncep C Column

Separation type: Liquid Chromatography Mixed-mode

HPLC Determination of CBD, CBN, THC in 1:1 Citrus Lime (100mg CBD/100mg THC) Tonic on Cannsep C Column
HPLC Determination of CBD, CBN, THC in 1:1 Citrus Lime (100mg CBD/100mg THC) Tonic on Cannsep C Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, delta-9 THC, delta-8 THC

Cannabidiol (CBD), cannabinol (CBN), and delta-9-Tetrahydrocannabinol (THC) are three of the most active cannabinoids in cannabis. THC is the strongest of the three and is the main cause of the psychoactive effects of cannabis. CBD, while the 2nd most active component, does not cause the same effects by itself, but studies show it may have other neurologic and psychiatric benefits. There has been a lot less research conducted on CBN and its potential health benefits, but it is a product of the breakdown of THC and works on the same receptors (albeit with different effects) as CBD.
These cannabinoids can be detected in the low UV regime. Using a Cannsep C reverse-phase column and a mobile phase consisting of water, methanol (MeOH) and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, CBD, CBN, and THC can be separated, measured, and analyzed. This analysis method can be UV detected at 210 nm with high resolution, even in real-world samples.

ColumnCannsep C, 2.1×100 mm, 5 µm, 100A
Mobile PhaseMeCN/MeOH/H2O – 40/35/25%
Flow Rate0.5 ml/min
DetectionUV, 210 nm
Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC),  Cannabinol (CBN)

Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

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Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-9-Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.