HPLC Determination of Δ8-THC, CBD, Δ9-THC, CBN, on Primesep N Column

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Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a normal-phase Primesep N column using a mobile phase consisting of hexane and ethyl acetate without the need for a buffer with UV detection at 275nm.


Column Primesep N , 3.2×150 mm, 3 µm, 100A
Mobile Phase Hexane/Ethyl Acetate – 97/3%
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 275 nm



Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds  Delta -8-Tetrahydrocannabinol, Δ8-THC, Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol, Δ9-THC, Cannabinol (CBN)


Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.