Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a normal-phase Primesep N column using a mobile phase consisting of hexane and ethyl acetate without the need for a buffer with UV detection at 275nm.
| Column | Primesep N , 3.2×150 mm, 3 µm, 100A |
| Mobile Phase | Hexane/Ethyl Acetate – 97/3% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 275 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Delta -8-Tetrahydrocannabinol, Δ8-THC, Cannabidiol (CBD), Delta-9-Tetrahydrocannabinol, Δ9-THC, Cannabinol (CBN) |
Application Column
Primesep N
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
